Research Article |
Corresponding author: Mark R. Shaw ( markshaw@xenarcha.com ) Academic editor: Gavin Broad
© 2015 Mark R. Shaw, Ian Sims.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Shaw MR, Sims I (2015) Notes on the biology, morphology, nomenclature and classification of Pseudavga flavicoxa Tobias, 1964 (Hymenoptera, Braconidae, Rhysipolinae), a genus and species new to Britain parasitizing Bucculatrix thoracella (Thunberg) (Lepidoptera, Bucculatricidae). Journal of Hymenoptera Research 42: 21-32. https://doi.org/10.3897/JHR.42.8935
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The solitary parasitoid Pseudavga flavicoxa has been reared, in some numbers, from cocoons of the bucculatricid moth Bucculatrix thoracella collected as larvae descending from Tilia × vulgaris to form their cocoons, at Jealott’s Hill, Berkshire, England. The taxonomic confusions and complications bedevilling its determination are outlined, and the recognition of the genus Pseudavga Tobias, 1964 is proposed. Egg placement in this koinobiont ectoparasitoid and the related genus Rhysipolis is discussed.
Cantharoctonus , Rhysipolis , Pachystigmus , France, Croatia
Among parasitoids of Lepidoptera reared during 2010 and 2011 by IS and sent to MRS for determination was a small (2.2 mm) predominantly straw-orange female specimen of a cyclostome braconid of the subfamily Rhysipolinae. It had been reared, probably in spring 2011 [found dead], from a cocoon of the bucculatricid moth Bucculatrix thoracella (Thunberg) collected at Jealott’s Hill, Berkshire, England on 13.ix.2010 as a larva descending from Tilia × vulgaris to spin up. The parasitoid was instantly recognised for what it was, as there are several similar specimens in the National Museums of Scotland (NMS) at the time provisionally standing over the incorrect name Rhysipolis rustus Papp, some of which were also reared from Bucculatrix in mainland Europe. However, no other British record or specimen had been seen, and nor had MRS been able to place them satisfactorily to genus within the tangled classification of Rhysipolinae. Further specimens reared from B. thoracella at Jealott’s Hill have subsequently been obtained.
As well as simply bringing forth and figuring this species as a British insect (Figures
In addition, some preliminary observations on the biology of P. flavicoxa are recorded, and both morphological and biological comparisons with Rhysipolis Foerster, 1862 are made. While the generic classification within Rhysipolinae remains in need of a wider review, morphological notes on the New World genus Cantharoctonus Viereck, 1912 are also given.
All B. thoracella that produced P. flavicoxa were collected as descending larvae at Jealott’s Hill, Berkshire, England (see “Biological observations” below). Experimental exposures involved wild B. thoracella larvae of various ages collected from Tilia × vulgaris in Edinburgh.
Figures
Following the rearing of a single ♀ from B. thoracella collected in 2011 (see above), IS attempted to rear further specimens from this host from the same Tilia × vulgaris trees in the autumn of 2012, collecting around 150 host larvae. However, owing to a communication failure, only approximately half-grown larvae were collected, and only moths resulted. The following year, from 4.ix–19.x.2013, 92 fully grown descending larvae produced 2 ♀, 1 ♂ of P. flavicoxa in x. 2013, and a further ♀ was found dead by iv.2014; otherwise practically all cocoons produced moths in 2014. In autumn 2014, in preparation for more detailed research on the biology of the parasitoid proposed for 2015, a large number of descending larvae were collected from the same Tilia trees in the period 9.ix–4.x.2014, and the resulting cocoons were kept indoors until being dispatched to Edinburgh, where they were received on 10.x.2014. By that date around 20 P. flavicoxa (both sexes equally) had emerged from the earliest made collections (9–10.ix.2014), but the remaining Bucculatrix cocoons were immediately placed in an outdoor rearing shed (cf.
Additional specimens, clearly congeneric with the British material and some reared from a further two species of Bucculatrix, are in NMS as follows:
CROATIA: 1 ♂, Oputija, ex Bucculatrix frangutella (Goeze), final instar larva coll. x.1988, em. x/xi.1988 (J.L. Gregory).
FRANCE: 1 ♀, Côte d’Or, Abbey de la Bussière, at light 21.vii.2003 (M.R. Shaw); 1 ♀, 1 ♂, Dordogne, La Barrière, 15 km S Riberac, at light 4–12.viii.2007 (M.R. Shaw;) and 1 ♀, same data but ex Bucculatrix ulmella on Quercus, coll. and em. viii.2007 (M.R. Shaw).
A specimen from France present in NMS that is very similar to (certainly congeneric and provisionally regarded as conspecific with) the British reared specimens had been determined by MRS in 2003 as the nominal taxon Rhysipolis rustus Papp, based on the original description (
In their attempt to clarify the application of the generic names Cantharoctonus, Noserus (now Pachystigmus, which has a different type species) and Pseudavga,
In
Sergey A. Belokobylskij (pers. comm.) has agreed with the present conclusion that Pseudavga is a genus distinct from Pachystigmus, regarding the reduced prepectal carina in Pseudavga, which is present only laterally [but variable; in some British specimens more extensive], as the most important difference. We have not seen the type of Pachystigmus nitidulus and consequently can offer no further opinion, but the original description (
However, whether or not Pseudavga should be retained as a genus distinct from the New World genus Cantharoctonus seems more doubtful, although in Cantharoctonus (to judge from the four undetermined N. American specimens in NMS) the complete prepectal carina appears to be much stronger than in Pseudavga flavicoxa, in which it is only weakly present.
Taken together, the available material of Pseudavga in NMS is very variable (e.g. in respect of colour, position of the radius on the pterostigma, shape and sculpture of the first metasomal tergite, detail of carination and sculpture of the propodeum, lengths of antennal segments and perhaps ovipositor sheath) and may represent more than one (possibly up to three) species. However, even though from a single population, there is enough variation in the British material (which must surely belong to only one species) to lead us to conclude that there is insufficient material at hand to assess possible species limits in the wider material in NMS, so provisionally we regard it as all belonging to a single variable species, P. flavicoxa.
The host of the British specimens, Bucculatrix thoracella, has greatly increased its range and abundance in Britain over the past 40 years, probably both through broadening its foodplant tolerance from Tilia cordata, a native but local and restricted woodland tree, to include the widely planted Tilia × vulgaris, and by becoming thoroughly plurivoltine (
One of the descending B. thoracella larvae intercepted by IS on 2.x.2014 was being grappled by a small orange insect which (although it escaped) was almost certainly P. flavicoxa, and inspection of some other descending larvae revealed the presence of single eggs, 0.2 mm long and in each case (7 observations) transversely placed (sub)dorsally along the intersegmental membrane behind the first thoracic segment (Figures
In Edinburgh, indoors at room temperature (18–22 °C), 1–2 mid-final instar host larvae were kept, on strips of Tilia × vulgaris leaf, in 2.5 × 7.5 cm corked glass tubes with a single (probably mated) female P. flavicoxa (6 replicates), with diluted honey smeared on the glass, from 13.x.2014 (after 3 days of feeding in the absence of hosts) until the females died (the last one on 25.x.2014). At times, hosts in moulting cocoons were also added. The hosts were inspected twice daily, and replaced as they formed cocoons; all cocoons were opened and the larvae within also inspected. No ovipositions resulted, and indeed (including during the initial period of inspection afforded to each female being newly introduced to hosts) no interest whatsoever in the hosts or their traces was observed (Figure
Although the act of oviposition per se has not been seen in either Rhysipolis or Pseudavga, there are some comparisons that can be drawn simply from observation of the eggs. In Rhysipolis the egg is similarly placed transversely across a host intersegmental membrane, though with much greater flexibility as to the segments concerned (
Studied Rhysipolis species (
We are grateful to Kees van Achterberg for his support, provision of literature and comments on a draft. Sergey A. Belokobylskij made several helpful comments in the course of refereeing the MS. John L. Gregory kindly donated the Croatian specimen to MRS for the NMS collection. Richard Lyszkowski produced the images for Figures