Species estimates differ among single year and multi-year bee sampling. Comparison of sample-sized-based rarefaction (solid lines) and extrapolation (dashed line) from the pan traps samples at permanent sites in 2001 and the samples made by netting and pan traps throughout the same area encompassed by the permanent sites over 9 years (the “core” area). Calculations here are for Hill numbers of order q=0; orders q = 1 and q = 2 are in Suppl. material 1: Figures S1–S3. Bee species richness at the base sample size of 42,798 individuals (i.e., double the smaller reference size) is 406.0 for samples from the core area and 321.8 from the 2001 pan traps. Although bees were sampled from the same area, differences in sampling and years sampled gave different species richness estimates.