Time invested by Atta cephalotes workers in prophylactic behavior during the basal period (0 h) the first hour, and the second hour after challenge with water (white boxes) or Metarizhium anisopliae spores (gray boxes) A self-grooming B allogrooming C fungus grooming, and D fecal fluid grooming. Each box represents the sum of the time in seconds invested in each behavior from 10 workers (n = 5 nests, 60 workers per nest). Different letters indicate significant differences (p < 0.05).