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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Barcode reference library coverage analyses
    • In silico primer evaluation
    • Sampling, identification and laboratory processing of specimens
    • Mock community design
    • Metabarcoding PCR, replication strategy, library preparation and sequencing
    • Quality filtering, MOTU clustering and taxonomic assignment
  • Results
    • Barcode coverage
    • Species delimitation congruence
    • In silico primer evaluation
    • Bioinformatic analysis of mock communities and detection rates
  • Discussion
    • Barcode coverage analysis
    • Congruence of MOTUs with morphospecies
    • Pipeline evaluation and potential error sources
    • Quantitative estimations from metabarcoding results: Is it possible?
    • Multiple MOTUs originating from single specimens
  • Conclusion
  • Acknowledgements
  • References
  • Supplementary materials
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