Research Article |
Corresponding author: Kazumu Kuramitsu ( kuramitsu.kazumu.ws@alumni.tsukuba.ac.jp ) Academic editor: Marko Prous
© 2016 Kazumu Kuramitsu, Atsuya Kosaki, Teruhito Ishihara, Hideo Yamada, Kyohei Watanabe.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Kuramitsu K, Kosaki A, Ishihara T, Yamada H, Watanabe K (2016) Infestation of the woodwasp Tremex apicalis Matsumura (Hymenoptera, Siricidae) on the large-leaf dogwood Swida macrophylla (Wall.) with biological notes on its parasitoid wasps. Journal of Hymenoptera Research 52: 71-79. https://doi.org/10.3897/jhr.52.10060
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The woodwasp Tremex apicalis (Hymenoptera: Siricidae) infesting a decayed stand of Swida macrophylla (Cornales: Cornaceae) was found in Honshu, Japan. S. macrophylla was newly recorded as a host tree of the woodwasps. We observed oviposition behavior of T. apicalis on the tree trunk on May, 2015. In addition, prepupae and pupae of T. apicalis were observed in the wood on April, 2016. However, no larvae of T. apicalis were found at that time. This suggests that T. apicalis requires one year from egg to pupation. Parasitoid wasps, Ibalia japonica (Hymenoptera: Ibaliidae) and Megarhyssa spp. (Hymenoptera: Ichneumonidae), were also observed on the trunk (oviposition behavior of adult females) and in the wood (pupae and newly emerged adults). Because Ibalia and Megarhyssa are known as larval parasitoids of woodwasps and there were no other insect species in the wood, we conclude that these wasps are parasitoids of T. apicalis larvae. These parasitoids appear to be major natural enemies of T. apicalis larvae in the study site.
Cerrena unicolor , horntail, Ibalia japonica , Ibaliidae , Ichneumonidae , Megarhyssa , oviposition behavior, wood borer, natural enemy
Woodwasps (= horntails) (Hymenoptera, Siricidae) are common forest pests with some species known as serious pests of trees. Some introduced woodwasps cause serious damage to forests, regardless of their extent of damage in the native habitat (
Tremex apicalis Matsumura is distributed in Japan (
In May 2015, we found a large-leaf dogwood, Swida macrophylla (Wall.) (Cornales: Cornaceae) infested by T. apicalis in Ibaraki, Honshu, Japan. We then observed the oviposition behavior of T. apicalis and its parasitoid wasps. Fortunately, we were able to obtain permission to split the wood, and observe the wasp biology inside the wood the next spring. The results of these observations provide new information on the host-parasitoid relationships and biology of T. apicalis and its parasitoids. This information may contribute to the protection of Japanese garden plants. In this paper, our observations on these species are described.
A field survey of T. apicalis was carried out at the Botanical Garden in the Agricultural and Forestry Research Center (36°07'10"N; 140°05'50"E (DMS), ca. 25 m a.s.l.), University of Tsukuba, Ibaraki Prefecture, Honshu, Japan. In the study site, we found one S. macrophylla tree with numerous emergence holes of T. apicalis in the trunk on May 6, 2015 (Fig.
We observed the target S. macrophylla tree from 11:30 to 12:30 on May 6, 2015. Species of hymenopteran insects that landed on the S. macrophylla tree and their behavior were recorded for 60 min. From the observation day until March 1, 2016, we observed the infested tree approximately once a week whether or not adult woodwasps visited.
The infested tree was felled with a chainsaw on March 8, 2016 and the wood kept outside. The infested part, total 14.725 kg wet wt with woodwasp emergence holes, was split using a 7.2 ton electric wood splitting machine (E’Z - Splitter IG-700A, Husqvarna Zenoah Co. Ltd) and a hatchet on April 10, 2016. Insect species and their stages were tallied.
Voucher specimens are deposited in the Laboratory of Applied Entomology and Zoology, University of Tsukuba (Tsukuba City, Japan) and Kanagawa Prefectural Museum of Natural History (Odawara City, Japan).
In the field survey, we observed a total of 10 T. apicalis (3 males, 7 females) and 24 parasitoid wasps, i.e. Ibalia japonica Matsumura (Hymenoptera: Ibaliidae), Megarhyssa jezoensis (Matsumura) and M. sp.1 (Hymenoptera: Ichneumonidae), that landed on the tree (Fig.
male | female | Total | |
---|---|---|---|
Tremex apicalis | 3 | 7 | 10 |
Ibalia japonica | 9 | 5 | 14 |
Megarhyssa spp. | 2 | 8 | 10 |
M. jezoensis female | - | 5 | 5 |
M. sp.1 female | - | 3 | 3 |
M. spp. male |
2 | - | 2 |
From the S. macrophylla wood, 35 prepupal or pupal T. apicalis and 16 pupal or adult parasitoids were obtained (Table
S. macrophylla wood infested with T. apicalis. a Wood discoloration in the cross section with T. apicalis infestation b Basidiocarp of Cerrena unicolor in the wood infested with T. apicalis c Longitudinal section through stem of the wood infested with T. apicalis and woodwasp larvae, pupae and larval tunnels. Scale lines = (a, c) 100 mm; (b)10 mm.
In this study, we observed oviposition behavior of T. apicalis on a S. macrophylla tree, and the presence of prepupae and pupae in the tree. The order Cornales, which includes S. macrophylla, is a newly recorded host of T. apicalis.
In a field survey, many ovipositors of T. apicalis were observed on the trunk of a S. macrophylla tree.
No T. apicalis adults were observed on the tree trunk from June 2015 until the following spring. Hence T. apicalis is univoltine, active mainly in the spring. After wood splitting, we observed prepupae and pupae of T. apicalis but no larvae in the wood. This suggests that T. apicalis takes almost one year to develop from egg to pupal stages. Since most of the collected prepupae died after wood splitting, we were unable to observe whether they would emerge within this year.
All T. apicalis prepupae and pupae were found in the discolored part of the wood and the wood had basidiocarps of Cerrena unicolor on the bark (Fig.
Hymenopteran parasitoids, I. japonica, M. jezoensis and Megarhyssa sp. 1, were also observed in/on T. apicalis infested S. macrophylla. We were unable to observe the larvae that parasitized T. apicalis larvae. However, all known ibaliids are primary solitary, koinobiont endoparasitoids of woodwasps (
Because Ibalia and Megarhyssa are known as solitary parasitoids, their proportion in the wood (Table
We thank the Agricultural and Forestry Research Center Botanical Garden, University of Tsukuba for permission to conduct this study including wood splitting. We also thank Dr. Akihiko Shinohara (National Museum of Nature and Science), Mr. Masato Ito (Kobe University), Mr. Tatsuro Konagaya (Kyoto University) and Mr. Jemin Park (Paichai University) for offering information on the woodwasp and parasitoid wasps and Dr. Yuichi Yamaoka (University of Tsukuba) and Dr. Tsutomu Hattori (Forestry and Forest Products Research Institute) for their help with species identification of Cerrena unicolor. We are grateful to Dr. Yooichi Kainoh, Dr. DeMar Taylor and Dr. Natsuko Kinoshita (University of Tsukuba) for helpful improvements in the manuscript.