Journal of Hymenoptera Research 26: 31–71, doi: 10.3897/JHR.26.2527
Revision of Zelodia (Hymenoptera, Braconidae, Agathidinae) from Thailand
Michael J. Sharkey 1,†, Stephanie A. C. Stoelb 1,‡
1 Department of Entomology, University of Kentucky, S225 Agricultural Science Center North, Lexington, KY 40546-0091, USA

Corresponding author: Michael J. Sharkey (msharkey@uky.edu); Stephanie A. Clutts (s.clutts@uky.edu)

Academic editor: Gavin Broad

received 9 December 2011 | accepted 27 April 2012 | Published 28 May 2012


(C) 2012 Michael J. Sharkey. This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


For reference, use of the paginated PDF or printed version of this article is recommended.

Abstract

The species of Thai Zelodia (Hymenoptera: Braconidae: Agathidinae) are revised. Twenty-one species are treated, 19 new species are described, i.e. Zelodia charoeni, Zelodia chongkraii, Zelodia cholathorni, Zelodia idrisi, Zelodia nikomi, Zelodia nopadoli, Zelodia pahangensis, Zelodia panyaii, Zelodia poonsathii, Zelodia ratanae, Zelodia saksiti, Zelodia surachaii, Zelodia suyaneeae, Zelodia toyae, Zelodia uthaii, Zelodia wangi, Zelodia wichaii, Zelodia wirati, Zelodia wirotei. A dichotomous key to species is presented; links to electronic interactive keys and to distribution maps are also included.

Keywords

Thailand, Insecta, identification key, taxonomy, systematics

Introduction

Agathidinae is a moderately large subfamily of Braconidae with 1, 061 described species worldwide and 238 in the Oriental Region (Yu et al. 2005) though there are an estimated 2, 000–3, 000 species awaiting description worldwide (Sharkey et al. 2006). The subfamily has a worldwide distribution and members are found in most terrestrial habitats. The history of higher classification of the Agathidinae was summarized by Sharkey (1992) who also proposed a tribal level classification based on ground-plan coding. Sharkey et al. (2006) conducted phylogenetic analyses based on morphology and the D2–3 regions of 28S rDNA. The Oriental fauna of Agathidinae was first revised by Bhat and Gupta (1977) and they provided a detailed history of taxonomic research for the area. Sharkey et al. (2009) revised the Oriental genera of Agathidinae. Achterberg and Long (2010) revised the Vietnamese agathidine fauna, erected the genus Zelodia, described 5 new species of Zelodia and transferred 20 species to Zelodia, mostly from Coccygidium and Zelomorpha. Sharkey and Clutts (2011) revised the Thai species of Biroia, Braunsia, Camptothlipsis, Coccygidium, Cremnops, Cremnoptoides, Disophrys, Earinus, Gyrochus, Lytopylus, and Troticus, and included a key to the Agathidinae genera of the Oriental region. This paper is the second in a series to revise all Thai species of Agathidinae. The genera Aneurobracon, Bassus, Euagathis, and Therophilus will be dealt with in subsequent publications.

Figure 1.

Map showing collection sites in Thailand.

Methods

As part of the inventory of Thai insects, we ran three Malaise traps at 30 different localities throughout Thailand from 2007-2010, comprising approximately 90 Malaise trap years. The specimens dealt with here are primarily from these traps.

Species concepts are based on morphological data and cytochrome c oxidase (COI) data. Phenetic and phylogenetic trees, using 558 base pairs of COI data, were constructed using neighbor-joining (NJ), maximum parsimony (MP) and Bayesian methods. MP was performed using TNT (Goloboff et al. 2008) [traditional search with 100 random addition sequences followed by branch-swapping, saving 100 trees per replication; 1000 bootstrap replications were used to estimate branch reliability]. The Bayesian analysis was performed using MrBayes v3.1.2 (Ronquist and Huelsenbeck 2003). Best-fitting DNA substitution models were determined using MrModeltest2.2 (Nylander 2004). The general time reversible model of evolution with a parameter for invariant sites and rate heterogeneity modeled under a gamma distribution (GTR+I+G) was determined as the best-fitting model. The Bayesian analysis consisted of two independent Bayesian MCMC runs initiated from different random starting trees. The analysis ran for 2, 000, 000 generations, reaching a topological similarity criterion of 0.01; trees were sampled every 500 generations. 25% of the trees from each run were removed as burn-in upon topological convergence. The NJ tree was produced from PAUP* (Swofford 2002) using default settings. Figure 2 presents the NJ tree, which was much more resolved than were the phylogenetic trees produced by MP and Bayesian analyses. We mapped the support values of the Bayesian and MP analyses on the NJ tree. Branches without values are those that collapsed in the phylogenetic analyses.

Figure 2.

NJ phylogram of the COI barcode region for 17 of the 21 Zelodia species treated here. Wherever Bayesian and parsimony analyses agreed with the NJ tree branch support values are included in the figure i.e., Bayesian posterior probabilities / parsimony bootstrap (bootstrap values less than 60 are not illustrated).

The dichotomous key, descriptions, and the interactive key (Appendices 1–3) were generated using DELTA Editor Dallwitz et al. (1999), DELTA Dallwitz et al. (1993), and Intkey Dallwitz et al. (1995).

Morphological terms follow Sharkey and Wharton (1997) except for the following: measurements are given for the length and apical width of the first metasomal mediotergite (MT1). Measurement of the apical width is straightforward, however since the base of the tergite is usually hidden from view it is difficult to measure the total length. Instead we measure from the apex of the large tendon that emanates from the propodeum and inserts near the base of the median tergite. Abbreviations used in text: S1, S2, S3; metasomal mediosternite 1, 2, 3; MT1, MT2, MT3: metasomal mediotergite 1, 2, 3; LT1, LT2, LT3: metasomal laterotergite 1, 2, 3.

Morphological terms used in this revision were matched to the Hymenoptera Anatomy Ontology (HAO, Yoder et al. 2010) (Appendix 4). Identifiers (URIs) in the format http://purl.obolibrary.org/obo/HAO_XXXXXXX represent anatomical concepts in HAO version http://purl.obolibrary.org/obo/hao/2011-05-18/hao.owl. They are provided to enable readers to confirm their understanding of the anatomical structures being referenced. To find out more about a given structure, including, images, references, and other metadata, use the identifier as a web-link, or use the HAO:XXXXXXX (note colon replaces underscore) as a search term at http://glossary.hymao.org.

All 19 species are treated with a diagnosis and distributional data. They are illustrated with color photos using a JVC digital camera mounted on a Leica MZ16 microscope and Automontage® stacking software. Distributional data are listed for all species and a Google map via Berkeley Mapper is included for all species. The descriptions are of the holotype and variation is given in parentheses.

The source files for the keys, descriptions, illustrations, DNA sequence and distributional data are all freely available to future researchers who may wish to build on this beginning. DNA trace files and primer information are available through the Barcode of Life Data system (BOLD) (Ratnasingham and Hebert 2007) at http://www.boldsystems.org. Sixteen of the twenty five Zelodia COI sequences were generated by BOLD (project ASTRK Revisions of Thai Agathidinae Braconids), the remaining nine were generated in the Sharkey lab. All sequences have been deposited in GenBank database (JQ763436–JQ763460). All twenty five Zelodia COI sequences are characterized by a -1 frameshift mutation. A majority of Agathidinae CO1 sequences are distinguished by a series of 1 bp deletions which are not restricted to one portion of the barcode region. Codon composition in sequences with 1bp deletions remains highly biased towards AT and substitutions remain biased towards 3rd codon position (M. Alex Smith pers. comm.). It is suggested that the genes are correctly decoded by a programmed frameshift during translation (Beckenbach et al. 2005) and are functional.

Distribution data, pdf’s of non-copyright references, images, notes, and host and type information can be found by searching TaxaBank (a combined specimen and taxonomic database; http://purl.org/taxabank). Codes beginning with an “H” and followed by numbers are unique identifiers used for specimens in the Sharkey lab at the University of Kentucky, and in the specimen database TaxaBank (e.g., H647).

Abbreviations used for specimen depositories are as follows:

BMNH The Natural History Museum, London, England.

FSCA Florida State Collection of Arthropods, Gainesville, Florida, USA.

HIC Hymenoptera Institute Collection, University of Kentucky, Department of Entomology, Lexington, Kentucky, USA.

QSBG Queen Sirikit Botanic Gardens, Chiang Mai, Thailand.

RMNH NCB Naturalis Collection [formerly Rijksmuseum van Natuurlijke Historie], Leiden, Netherlands.

UKM Universiti Kebangsaan, Bangi, Selangor, Malaysia.

Results

Refer to the tree in Sharkey and Clutts (2010) for generic level placement. The host lepidopterans of the genus are unknown. The neighbor joining branching diagram in Figure 2, based on COI mtDNA, was used to help in determining species limits although we did not devise any cutoff threshold to delimit species. We conducted Bayesian and parsimony analyses and where these agreed with the NJ tree the support values are given in Figure 2. An examination of this figure shows that the NJ diagram and the phylogenetic analyses were in close agreement. We also used morphological differences to delimit species. For example, Zelodia saksiti and Zelodia charoeni are very similar (1/558 bp difference) in COI sequences but are very different morphologically. Contrastingly, Zelodia wangi is a widespread species and COI sequences show some variation (2/558 bp difference), however we could find no morphological differences. The complex may represent several species.

Taxonomy Diagnosis

Members of Zelodia may be distinguished from all other Agathidinae with the following combination of characters: ovipositor short, shorter than length of metasoma; tarsal claws cleft; hind trochantellus with 2 longitudinal carinae; frons lacking lateral carinae between antennae and lateral ocelli.

Key to Thai species of Zelodia

(Note: there are often more than 2 alternatives in each couplet)

1 a. Hind femur all pale 2
b. Hind femur all melanic 7
c. Hind femur melanic dorsally and ventrally, orange laterally and medially Zelodia ratanae Sharkey sp.n.
2(1) a. Hind leg including tarsus entirely pale Zelodia brevifemoralis Achterberg & Long
b. Hind leg melanic and pale, often mostly pale with tarsus melanic 3
3(2) a. Mesoscutum entirely pale 4
b. Mesoscutum mostly melanic except margins pale Zelodia idrisi Sharkey sp.n.
4(3) a. Stigma entirely melanic Zelodia nikomi Sharkey sp.n.
b. Stigma partly or entirely yellow 5
5(4) a. Median ocellus distinctly narrower than space between lateral ocelli 6
b. Median ocellus about as widen as space between lateral ocelli Zelodia toyae Sharkey sp.n.
6(5) a. Hind femur more than 4× longer than wide (average 4.2×) Zelodia suyaneeae Sharkey sp.n.
b. Hind femur less than 4× longer than wide (average 3.5×) Zelodia wichaii Sharkey sp.n.
7(1) a. Mesoscutum entirely pale 8
b. Mesoscutum entirely melanic 10
c. Mesoscutum mostly pale with melanic spots posteriorly Zelodia chongkraii Sharkey sp.n.
d. Mesoscutum mostly melanic except on margins pale 12
8(7) a. Median tergite 1 mostly yellow 15
b. Median tergite 1 all white or cream colored, sometimes with a bit of melanic color subapically Zelodia wirotei Sharkey sp.n.
c. Median tergite 1 all black 9
9(8) a. Head (not including mouthparts or antenna) mostly or entirely pale Zelodia longidorsata (Bhat & Gupta)
b. Head (not including mouthparts or antenna) mostly or entirely melanic Zelodia panyaii Sharkey sp.n.
10(7) a. Median tergite 1 all black Zelodia cholathorni Sharkey sp.n.
b. Median tergite 1 all white or cream colored or sometimes with a bit of melanic color subapically 11
11(10) a. Head (not including mouthparts or antenna) mostly or entirely melanic Zelodia surachaii Sharkey sp.n.
b. Head (not including mouthparts or antenna) mostly or entirely pale Zelodia nopadoli Sharkey sp.n.
12(7) a. Propodeum melanic in anterior half or more 13
b. Propodeum entirely pale 14
13(12) a. Hind leg entirely melanic Zelodia poonsathii Sharkey sp.n.
b. Hind leg melanic except tibia mostly pale Zelodia saksiti Sharkey sp.n.
14(12) a. Fore wing not lighter apically than subapically, lacking white setae apically Zelodia uthaii Sharkey sp.n.
b. Fore wing lighter apically than subapically, with extensive white setae apically Zelodia pahangensis Sharkey sp.n.
15(8) a. Hind tibia entirely melanic Zelodia wangi Sharkey sp.n.
b. Hind tibia mostly pale 16
16(15) a. Median ocellus distinctly narrower than space between lateral ocelli Zelodia charoeni Sharkey sp.n.
b. Median ocellus about as wide as space between lateral ocelli Zelodia wirati Sharkey sp.n.
Species treatments
Zelodia brevifemoralis Achterberg & Long

http://species-id.net/wiki/Zelodia_brevifemoralis

Fig. 3
Zelodia brevifemoralis Achterberg & Long, 2010 [Holotype ♀ (RMNH) examined]
Diagnosis.

Hind leg, including tarsus (except apical tarsomere), entirely pale.

Figure 3.

Zelodia brevifemoralisAchterberg and Long a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1–MT2.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H334/ATRMK197-11/JQ763436.

Distribution.

Recorded from Vietnam and central Thailand.Distribution map of the sole Thai locality can be found at http://purl.org/thaimap/brevifemoralis

Material examined.

H0334 [QSBG] ♀, Thailand, Phu Kradueng NP, 420, 16.844°N, 101.692°E, MT, 1–7.vii.2008.

Diagnosis.

Hind tibia pale except apex black; hind femur melanic; mesoscutum pale; median ocellus distinctly narrower than space between lateral ocelli.

Description.

Body length 7.4 mm (7.2–7.4). Head. 41 (39–41) flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate apically, veins yellow basally except costa mostly melanic, melanic apically and around parastigma, stigma yellow in basal fifth. Hind tarsal claw bifid. Length/width of hind femur 2.22/0.552 = 3.9 (3.9–4.1). Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.257/0.539 = 2.3. Ratio of widest point of MT1 to narrowest point 0.539/0.35 = 1.5 (1.4–1.5). Color. Mostly yellow; black as follows: antenna except scape brown medially, hind leg except basal 4/5 of tibia yellow. Median area of mesopleuron yellow (to mostly brown).

Figure 4.

Zelodia charoeni sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H462/ATRMK214-11/JQ763437.

Distribution.

Found only in northern Thailand.Distribution map can be found at http://purl.org/thaimap/charoeni

Etymology.

Dedicated to Mr. Charoen Wanna, collector at Doi Phuka National Park.

Material examined.

Holotype ♀. H5905 [QSBG], Thailand, Doi Phahompok NP Headquarters, 569, 19.966°N, 99.156°E, MT, 25.vii–1.viii.2007.

Paratypes. Thailand: ♀, Nam Nao NP, Check point, 921m, 16.728°N, 101.563°E, MT, 28.iv–5.v.2007: H0461 [HIC]; ♂, Doi Phahompak Office, 569m, 20.191°N, 99.248°E, MT, 28.v–7.vi.2008: H0462 [QSBG].

Diagnosis.

Mesoscutum entirely melanic; hind tibia all melanic; head melanic; MT1 black.

Description.

Body length 6.0 mm. Head. 38 flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex densely and heavily punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron densely covered with setae. Fore wing cells weakly infuscate, more so apically, veins brown to black. Hind tarsal claw with elongate basal tooth. Length/width of hind femur 1.8/0.7 = 2.7. Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 0.91/0.659 = 1.37. Ratio of widest point of MT1 to narrowest point 0.7/0.4 = 1.5. Color. Black except palpi mostly yellow and metasomal laterotergites and S1–S3.

Figure 5.

Zelodia cholathorni sp.n. a lateral habitus b wings c anterodorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1–MT3.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H035/ATRMK141-09/JQ763438.

Distribution.

Found only at the type locality in northern Thailand.Distribution map can be found at http://purl.org/thaimaps/cholathorni

Etymology.

Dedicated to Mr. Cholathorn Chamnanthip, chief of Doi Phahompok National Park, one of our collecting sites.

Material examined.

Holotype ♂. H0035 [QSBG], Thailand, Doi Chiang Dao WS, Nature Trail, 491m, 19.403°N, 98.922°E, MT, 31.vii–7.viii.2007.

Paratypes. ♂ Thailand, Doi Chiangdao NP, Headquarters, 491m, 19.405°N, 98.922°E, MT, 21–28.viii.2007: H4597 [QSGB], H4593 [HIC].

Diagnosis.

Mesoscutum mostly pale with melanic spots posteriorly.

Description. Body length 7.3 mm (7.1–7.3). Head. 41 (40–41) flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells all weakly infuscate, more so apically, veins melanic. Hind tarsal claw bifid. Length/width of hind femur 2.169/0.506 = 4.3. Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.258/0.458 = 2.3 (2.2–2.3). Ratio of widest point of MT1 to narrowest point 0.543/0.405 = 1.3 (1.3–1.4). Color. Yellow, black, and white; metasomal segments 1 and 2, LT3 and S3 all white; yellow as follows: head except antenna, prothorax, most of mesoscutum and scutellar disc, fore leg, mid leg except coxa, trochanter, trochantellus, and base of femur partly melanic; remaining body parts black.

Figure 6.

Zelodia chongkraii sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H905/ATRMK260-11/JQ763439; H996/ATRMK273-11/JQ763440.

Distribution.

Eastern and northeastern Thailand.Distribution map can be found at http://purl.org/thaimap/chongkraii

Etymology.

Dedicated to Mr. Chongkrai Worapongthorn, chief of Doi Inthanon National Park, one of our collecting sites.

Material examined.

Holotype ♀. H0996 [QSBG], Thailand, Huai Nam Dang NP, Thung Buatong, 19.293°N, 98.6°E, MT, 21–28.iv.2008.

Paratype. ♀, Thailand, Kaeng Krachan NP, Panernthung/km27, 950m, 12.8217°N, 99.371°E, MT, 8–15.vi.2009, H0905 [HIC].

Diagnosis.

Head melanic dorsally and posteriorly, pale ventrally and anteriorly; hind femur all pale.

Description.

Body length 9.0 mm (7.6–9.0). Head. 43 (42–43) flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells yellowish basally, infuscate in distal 3rd fourth and clear in apical fourth with white setae, veins yellow basally and apically, brown at midlength, stigma yellow in basal fourth, otherwise dark brown. Hind tarsal claw bifid. Length/width of hind femur 2.63/0.75 = 3.5 (3.5–3.7). Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.60/0.82 = 2.0 (1.9–2.0). Ratio of widest point of MT1 to narrowest point 0.82/0.49 = 1.7 (1.5–1.7). Color. Mottled yellow, brown, orange, cream-colored, and black.

Figure 7.

Zelodia idrisi sp.n. a lateral habitus b dorsal head c lateral head and mesosoma d wings with buffered light source e wings with normal light source f dorsal head and mesosoma g dorsal propodeum h dorsal MT1–MT3.

Distribution.

Widespread in western Malaysia and likely present in southern Thailand.Distribution map can be found at http://purl.org/thaimap/idrisi

Etymology.

Dedicated to Prof. Idris Abd. Ghani, professor at the School of Environmental and Natural Resource Science, Universiti Kebangsaan, Malaysia.

Material examined.

Holotype ♀. H6733 [UKM], Malaysia, Kuala Lompat, Pahang, 3.695°N, 102.224°E, 3.iii.2009.

Paratypes. ♀, Malaysia: Kampung PETA, Johor, 2.541°N, 103.418°E, viii.2008: H8467 [QSBG]; Kuala Lompat, Pahang, 3.695°N, 102.224°E, 3.iii.2009:H8468 [QSBG]; Wang Kelian, Perlis, 6.678°N, 100.19°E, xi.2008: H8469 [HIC], H8466 [UKM]; Pasoh Forest Reserve, 23.x.2002: H8470 [HIC].

Zelodia longidorsata (Bhat & Gupta)

http://species-id.net/wiki/Zelodia_longidorsata

Fig. 8
Zelodia longidorsata (Bhat and Gupta) 1977 [Holotype, ♀ (CNC) examined]
Diagnosis.

Hind tibia all melanic; mesoscutum entirely pale; stigma entirely melanic; head, except antenna, pale; propodeum mostly or entirely pale; median ocellus diameter equal to the space between lateral ocelli; MT1 long and narrow, not much wider apically than basally

Distribution.

Recorded from southern India and a locality east of Bangkok, undoubtedly more widespread.Distribution map of Thai locality can be found at http://purl.org/thaimap/longidorsata

Figure 8.

Zelodia longidorsata a lateral habitus b dorsal head c lateral head and mesosoma d wings e dorsal mesosoma f dorsal propodeum and metasomal terga 1 and 2.

Material examined.

♀, Thailand, Chanda Buri, Pong Nam Ron, 12.908°N, 102.262°E, MT, 20-31.iii.2001: H8471 [RMNH].

Diagnosis.

Hind femur all pale; stigma entirely melanic; MT1 long and narrow, only slightly wider apically than basally.

Description.

Body length 5.5 mm. Head. 35 flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells with a yellowish hue basally, infuscate apically, veins yellow basally, melanic apically, stigma entirely melanic. Hind tarsal claw bifid. Length/width of hind femur 1.83/5.0 = 3.7. Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 0.95/0.585 = 1.6. Ratio of widest point of MT1 to narrowest point 0.585/0.394 = 1.5. Color. Mostly yellow except as melanic as follows: flagellum, lateral surface of scape, hind tarsus, apex of hind tibia.

Figure 9.

Zelodia nikomi sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1-MT3.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H518/ATRMK220-11/JQ763441.

Distribution.

Known only from the type locality in central Thailand.Distribution map can be found at http://purl.org/thaimap/nikomi

Etymology.

Dedicated to Mr. Nikom Wongwan, collector at Phuka National Park.

Material examined.

Holotype ♀. H0518 [QSBG], Thailand, Phu Kradueng NP, 420m, 16.844°N, 101.692°E, MT, 9–16.iii.2009.

Diagnosis.

Mesoscutum entirely melanic; head, except antenna, pale.

Description.

Body length 7.7 mm (6.2–7.7). Head. (37–41) flagellomeres (both flagella broken in holotype). Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron with moderately dense setae. Fore wing cells hyaline basally, infuscate distally, veins including stigma melanic. Hind tarsal claw bifid. Length/width of hind femur 2.059/0.551 = 3.8 (3.5–4.8). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.231/0.583 = 2.1 (2.1–2.3). Ratio of widest point of MT1 to narrowest point 0.583/0.376 = 1.6 (1.2–1.6). Color. Mostly black with yellow, white and cream color; head yellow, scape brown, flagellum black, prothorax yellow; fore leg and mid leg, except parts of coxa, yellow; MT1 and MT2 cream colored; basal laterotergites and sterna white; remainder of body black.

Figure 10.

Zelodia nopadoli sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1.

Distribution.

Recorded from southeastern and northwestern Thailand.Distribution map can be found at http://purl.org/thaimap/nopadoli

Etymology.

Dedicated to Mr. Nopadol Nachin, chief of Tad Tone National Park.

Material examined.

Holotype ♀. H0323 [QSBG], Thailand, Khao Khitchakut NP, N/Prabaht Unit, 500m, 12.816°N, 102.152°E, MT, 1–8.ix.2008.

Paratypes. ♀, Thailand: Doi Inthanon NP, Chiang Mai, 1700m, 18.544°N, 98.525°E, MT, 3-12.v.1900: H8491[HIC]; Doi Chiangdao NP, Pha Tang substation, 526m, 19.416°N, 98.9147°E, MT, 28.viii–4.ix.2007: H0948 [QSBG].

Diagnosis.

Mesoscutum mostly melanic except margins pale; hind tibia mostly pale except apex and extreme base black; hind femur melanic; apex of fore wing hyaline with white setae.

Description.

Body length 7.8 mm (7.4–7.8). Head. 39 (39–42) flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells weakly infuscate, veins mostly brown, lighter basally and apically, stigma yellowish brown in basal 1/3, apex of wing with white setae, making it appear lighter. Hind tarsal claw bifid. Length/width of hind femur 2.32/0.5 = 4.6 (4.5–4.6). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.3/0.59 = 2.2 (2.1–2.2). Ratio of widest point of MT1 to narrowest point 0.59/0.38 = 1.6 (1.5–1.6). Color. Mottled yellow, cream-colored and black; head yellow except vertex brown and antenna black, hind leg black except most of tibia pale.

Figure 11.

Zelodia pahangensis sp.n. a lateral habitus b dorsal head c lateral head and mesosoma d apex of fore wing e wings f dorsal head and mesosoma g dorsal propodeum h dorsal MT1–MT3.

Distribution.

Known only from the type locality in western Malaysia but likely to be found in peninsular Thailand.Distribution map can be found at http://purl.org/thaimap/pahangensis

Etymology.

The name is in reference to the Malaysian state, Pahang, in which the specimens were collected

Material examined.

Holotype ♀, H8489 [UKM], Malaysia, Pahang, Cameron Highland, Parit Falls, 4.493°N, 101.389°E, MT, i.2009.

Paratypes. ♂ Malaysia, Pahang, Cameron Highland: Jasa, 4.493°N, 101.389°E, MT, 28.ii.2009: H8482 [HIC], H8484 [HIC], H8485 [QSBG], H8488 [UKM]; Parit Falls, 4.493°N, 101.389°E, MT, i.2009: H8486 [QSBG], H8487 [QSBG], H8483 [HIC], H8465 [UKM].

Diagnosis.

Head (not including mouthparts) melanic; mesoscutum entirely pale.

Description.

Body length 7.5 mm. Head. 39 flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron densely covered with setae. Fore wing cells weakly infuscate, more so apically, veins melanic. Hind tarsal claw bifid. Length/width of hind femur 2.117/0.539 = 3.9. Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.234/0.505 = 2.4. Ratio of widest point of MT1 to narrowest point 0.505/0.421 = 1.2. Color. Black, reddish orange, brown, cream colored and white: mostly black, reddish orange as follows: pro and mesothorax, most of fore leg; mid leg mostly brown; fore and mid tarsi yellow; mouthparts cream colored and yellow, anterior laterotergites and sterna white.

Figure 12.

Zelodia panyaii sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1 and MT2.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H918/ATRMK263-11/JQ763442.

Distribution.

Known only from the type locality in northwestern Thailand.Distribution map can be found at http://purl.org/thaimap/panyaii

Etymology.

Dedicated to Mr. Panya Kotesanlee, chief of Phuphan National Park.

Material examined.

Holotype ♀ H0918 [ QSBG], Thailand, Doi Chiangdao NP, Pha Tang substation, 19.416°N, 98.9147°E, MT, 15-21.v.2008.

Diagnosis.

Mesoscutum mostly melanic except margins pale; hind tibia all melanic.

Description.

Body length. 7.2 mm. Head. 39 flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells weakly infuscate, veins mostly brown to black, M+Cu yellow. Hind tarsal claw bifid. Length/width of hind femur 2.059/0.586 = 3.5. Metasoma. Length/width ratio of MT1, 1.273/0.656 = 1.9. Ratio of widest point of MT1 to narrowest point 0.656/0.436 = 1.5. Color. Mottled yellow, cream-colored and black, head yellow except flagellum black and scape brown, hind leg black, MT1 and MT2 cream colored.

Figure 13.

Zelodia poonsathiti sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H647/ATRMK231-11/JQ763443.

Distribution.

Found only at the type locality in southern peninsular Thailand.Distribution map can be found at http://purl.org/thaimap/poonsathiti

Etymology.

Dedicated to Mr. Poonsathit Wongsawat, chief of Doi Phuka National Park.

Material examined.

Holotype ♂ H0647 [QSBG], Thailand, Khao Sok NP, Bang Huaraed, 122m, 8.909°N, 98.509°E, MT, 11-18.xi.2008.

Diagnosis.

Hind tibia mostly pale, extreme base cream, apex black, most of dorsal and ventral surfaces black, otherwise orange.

Description.

Body length 7.6 mm. Head. 42 flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex densely and heavily punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells and veins melanic in basal 3/5, cells hyaline basally with white setae, veins R1, RS, r-m, and M yellow distally, stigma yellow in apical third. Hind tarsal claw bifid. Length/width of hind femur 2.4/0.8 = 3.2. Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.112/1.245 = 0.88. Ratio of widest point of MT1 to narrowest point 1.2/0.4. Color. Black except as follows; scape yellow, margins of prothorax cream colored to yellow, legs with extensive orange and/or yellow, scutellar sulcus, lateral and posterior areas of scutellum, and much of metapectal-propodeal complex, yellowish orange, anterior laterotergites and sterna pale brown.

Figure 14.

Zelodia ratanae sp.n. a lateral habitus b dorsal head c lateral head and mesosoma d wings e dorsal head and mesosoma f dorsal propodeum g dorsal metasoma.

Molecular data.

TaxaBank#/Genbank Accession: H273 /JQ763452.

Distribution.

Found only at the type locality in southern peninsular Thailand.Distribution map can be found at http://purl.org/thaimap/ratanae

Etymology.

Dedicated to Ms. Ratana Luckanawarakul. She is currently the director of the Recreation and Interpretation Division of the Thai Department of National Parks. Ratana was of great assistance to us on numerous field trips and was always the best of company.

Material examined.

Holotype ♂. H0273 [QSBG], Thailand, Khao Pu-Khao Ya NP, 75m, 7.551°N, 99.789°E, MT, 8–10.v.2006.

Diagnosis.

Mesoscutum mostly melanic except margins pale; hind tibia mostly pale except apex black; propodeum color mostly or entirely melanic.

Description.

Body length 8.4 mm (7.2–8.4). Head. 41 (40–41) flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells weakly infuscate, veins melanic. Hind tarsal claw bifid. Length/width of hind femur 2.007/0.533 = 3.8 (3.8–4.0). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.318/0.62 = 2.1 (2.0–2.1). Ratio of widest point of MT1 to narrowest point 0.620/0.389 = 1.6 (1.6–1.7). Color. Mottled yellow, orange, cream-colored and black, head yellow except antenna black, hind leg black except most of tibia pale.

Figure 15.

Zelodia saksiti sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1-MT3.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H036/ATRMK142-09/JQ763444.

Distribution.

Widespread throughout western Thailand.Distribution map can be found at http://purl.org/thaimap/saksiti

Etymology.

Dedicated to Saksit Poonsapsiri, one of the chiefs of Phataem National Park.

Material examined.

Holotype ♀. H0324 [QSBG], Thailand, Khao Sok NP, Head quarters, 115m, 8.915°N, 98.53°E, MT, 12-19.v.2009.

Paratypes. ♂, Thailand: Salaeng Luang NP: 557m, 16.844°N, 100.882°E, MT, 25.viii–1.ix.2006: H0036 [QSBG], H8464 [UKM]; 479m, 16.839°N, 100.863°E, MT, 11–18.viii.2006: H5996 [QSBG]; ♀, Khao Sok NP, Headquarters, 155m, 8.915°N, 98.53°E, MT, 9–16.vi.2009: H0663 [HIC]; ♂, evergreen forest, MT, 1988: H8490[BMNH]; ♀, Kaeng Krachan NP, km16/road/stream2, 320m, 12.802°N, 99.444°E, MT, 25.v–1.vi.2009: H0920 [HIC]; ♀, Doi Chiangdao NP: Headquarters, 491m, 19.405°N, 98.922°E, MT, 18–25.ix.2007: H4108 [QSBG]; Pha Tang substation, 526m, 19.416°N, 98.915°E, MT, 28.viii–4.ix.2007: H5967 [UKM].

Diagnosis.

Mesoscutum entirely melanic; hind tibia all melanic; head (not including mouthparts or antenna) melanic; apex of flagellum pale, contrasting with melanic base.

Median tergite 1 white or cream colored.

Description.

Body length 6.6 mm (6.6–8.3). Head. 41 flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron with moderately dense setae. Fore wing cells weakly infuscate, veins and stigma melanic. Hind tarsal claw bifid. Length/width of hind femur 2.019/0.511 = 4.0 (4.0–4.7). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.117/0.518 = 2.2 (2.2–2.3). Ratio of widest point of MT1 to narrowest point 0.518/0.427 = 1.2 (1.2–1.4). Color. Black except mouthparts yellow, apical 12 flagellomeres yellowish brown, MT1 cream colored, L1 and L2 and basal sterna white.

Figure 16.

Zelodia surachaii sp.n. a lateral habitus b wings c dorsal head d dorsolateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1-MT2.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H389/ATRMK207-11/JQ763445.

Distribution.

Taiwan, peninsular Malaysia and Thailand.Distribution map can be found at http://purl.org/thaimap/surachaii

Etymology.

Dedicated to Mr. Surachai Pransil, chief of Khao Kitchagoot National Park.

Material examined.

Holotype ♀. H0389 [QSBG] Thailand, Kaeng Krachan NP, Panernthung/km27, 950m, 12.819°N, 99.375°E, MT, 18–25.vii.2008.

Paratypes. ♀, Malaysia, Cameron Highlands, Tanah Rata, 4.475°N, 101.38°E, MT, iii–iv.2001: H1122 [UKM]; ♂, Taiwan, Taipei Co., Chuntou, 9.iv.1999: H8492 [FSCA].

Diagnosis.

Hind femur all pale; mesoscutum entirely pale; hind tibia mostly pale except apex and sometimes extreme base black; stigma partly or entirely yellow; median ocellus distinctly narrower than space between lateral ocelli; lateral surface of hind femur sparsely punctate, spaces between punctures wider than diameter of punctures.

Description.

Body length 5.7 mm (5.7–6.4). Head. 40 (40–45) flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate apically, veins yellow basally, melanic apically and around parastigma, stigma yellow in basal third. Hind tarsal claw bifid. Length/width of hind femur 1.856/0.531 = 3.5 (3.4–4.4). Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 0.87/0.434 = 2.0 (2.0–2.5). Ratio of widest point of MT1 to narrowest point 0.434/0.269 = 1.3 (1.3 – 1.4). Color. Yellow except as follows: flagellum melanic, lateral surface of scape brown, apex of hind tibia and entire tarsus melanic.

Figure 17.

Zelodia suyaneeae sp.n. a lateral habitus b wings c dorsal head d dorsolateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and metasoma.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H1603/ATRMK321-11/JQ763446; H383/ATRMK206-11/JQ763447.

Distribution.

Widespread in northern and central Thailand.Distribution map can be found at http://purl.org/thaimap/suyaneeae

Etymology.

Dedicated to Dr. Suyanee Vessabutr. She was the deputy director of QSBG and was of great assistance to us in providing space and resources at the museum.

Material examined.

Holotype ♀. H1603 [QSBG], Thailand, Chae Son NP, Visitor center, 18.836°N, 99.4739°E, MT, 15-22.ix.2007.

Paratypes. ♀, Thailand, Khuean Srinagarindra NP, 210m, 14.5°N, 98.884°E, MT, 26.iii-2.iv.2009: H0355 [QSBG], H0359 [QSBG]; 22–29.i.2009: H0383 [HIC]; Chae Son NP, Nature Trail; 507m, 18.836°N, 99.473°E, MT, 14–21.x.2007: H4103 [HIC]; 1–8.x.2007: H4119 [HIC]; Doi Chiangdao NP, Headquarters, 491m, 19.405°N, 98.922°E, MT: 18–25.ix.2007: H4113 [HIC]; 19.404°N, 98.9218°E, MT, 21–27.v.2008: H0891 [UKM]; Phu Kradueng NP, 274m, 16.942°N, 101.694°E, MT, 26.iv–3.v.2008: H0572 [UKM]; Kaeng Krachan NP, km16/road/stream2, 320m, 12.802°N, 99.444°E, MT, 25.v–1.vi.2009: H0921 [UKM]; ♂, Phu Phan NP, 526m, 16.81°N, 103.891°E, MT, 16–22.vi.2007: H0293 [QSBG].

Diagnosis.

Hind femur all pale; mesoscutum entirely pale; hind tibia mostly pale except apex black; median ocellus about as wide as or wider than space between lateral ocelli; stigma partly yellow.

Description.

Body length 7.6 mm. Head. 41 flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate apically, veins yellow basally except costa mostly melanic, veins melanic apically and around parastigma, stigma yellow in basal third. Hind tarsal claw bifid. Length/width of hind femur 2.25/0.54 = 4.2. Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.28/0.59 = 2.2. Ratio of widest point of MT1 to narrowest point 0.59/0.325 = 1.8. Color. Yellow except as follows: flagellum melanic, lateral surface of scape brown, apex of hind tibia and entire tarsus melanic.

Figure 18.

Zelodia toyae sp.n. a lateral habitus b wings c dorsal head d lateral head and mesosoma e dorsal head and mesosoma f dorsal propodeum g dorsal metasoma.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H381/ATRMK205-11/JQ763448.

Distribution.

Known only from the type locality in northwestern Thailand.Distribution map can be found at http://purl.org/thaimap/toyae

Etymology.

Dedicated to Ms. Chayanit (Toy) Satatha. Toy was the sorter of Diptera for the TIGER project and is currently a technician at QSBG. The species name reflects her nick-name, Toy.

Material examined.

Holotype ♀. H0381 [QSBG], Thailand, Namtok Mae Surin NP, Car park, 19.36°N, 97.988°E, MT, 27.iv–4.v.2008.

Diagnosis.

Mesoscutum mostly melanic except margins pale; hind tibia mostly pale except apex and extreme base black; hind femur all melanic; apical half of wing not noticeably more infuscate than base.

Description.

Body length. 6.1 mm. Head. 42 flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate distally, veins yellow basally except costa black, veins melanic distally, stigma melanic. Hind tarsal claw bifid. Length/width of hind femur 2.05/0.54 = 3.8. Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.13/0.61 = 1.9. Ratio of widest point of MT1 to narrowest point 0.61/0.38 = 1.6. Color. Mottled yellow, cream-colored and black, head yellow except antenna black, hind leg black except most of tibia pale.

Figure 19.

Zelodia uthaii sp.n. a lateral habitus b wings c anterior head d dorsal head e lateral head and mesosoma f dorsal head and mesosoma g dorsal propodeum h dorsal MT1–MT3.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H309/ATRMK450-11/JQ763449.

Distribution.

Known only from the type locality in western Thailand.Distribution map can be found at http://purl.org/thaimap/uthaii

Etymology.

Dedicated to Mr. Uthai Promnaree, one of the chiefs of Phataem National Park.

Material examined.

Holotype ♀. H0309 [QSBG], Thailand, Mae Wong NP, Chong Yen, 1306m, 16.087°N, 99.11°E, MT, 24.ix–1.x.2007.

Diagnosis.

Scutum and MT1 yellow; hind leg, including tibia, melanic.

Description.

Body length 6.7 mm (6.5–7.1). Head. 43 (41–45) flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells very weakly infuscate with a yellowish tinge basally, veins yellow basally, melanic in apical half, stigma yellow in basal fourth (or entirely melanic). Hind tarsal claw bifid. Length/width of hind femur 2.0/0.5 = 3.9 (3.7–3.9). Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.156/0.481 = 2.4 (2.4–2.5). Ratio of widest point of MT1 to narrowest point 0.481/0.349 = 1.4 (1.4). Color. Yellow except as follows: scape and pedicel pale brown, flagellum brown, hind leg mostly brown in females with areas near joints and most of tibia pale brown to yellow, anterior metasomal laterotergites and sterna cream-colored, median tergites entirely yellow in holotype (but some female paratypes have pale brown markings on apical tergites; males as in females but with hind leg mostly to entirely black, and apical terga and sterna extensively black).

Figure 20.

Zelodia wangi sp. n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum h dorsal MT1–MT3.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H074/JQ763457; H023/JQ763455; H073/JQ763456; H019/JQ763458; H025/JQ763460; H084/JQ763459.

Distribution.

Widespread throughout Thailand. Distribution map can be found at http://purl.org/thaimap/wangi

Etymology.

Dedicated to Mr. Wang Saeyang, who was the sorter for Hymenoptera for the TIGER project.

Material examined.

Holotype ♀. H0057 [QSBG], Thailand, Khoa Chong, Forest Research Stn., 75m, 7.551°N, 99.789°E, MT, vii–xi.2005.

Paratypes. ♀, Thailand: Khoa Chong, Forest Research Stn., 75m, 7.551°N, 99.789°E, MT, xi.2005: H0019 [QSBG], H5990 [UKM], H0084 [HIC]; x.2005: H0023 [QSBG]; viii.2005: H0054 [QSBG], H5906 [UKM], H5988 [UKM], H5991 [UKM], H5994 [UKM], H0061 [UKM]; ♂, x.2005: H5992 [UKM]; viii.2005: H5986 [UKM]; ♀, Thailand, Nayong Khaochong, 75m, 7.561°N, 99.886°E, MT, 24–27.vi.2005: H0025 [QSBG], H0034 [QSBG]; ♀, Nayong Khaochong, 75m, 7.561°N, 99.886°E, MT, 15–18.vi.2005: H0074 [HIC]; 6-8.vii.2005: H0076 [HIC]; ♂, 26–30.vii.2005: H0073 [HIC]; ♀, Khao Sok NP, Headquarters, 115m, 8.915°N, 98.53°E, MT, 12–19.v.2009: H0391 [QSBG]; 17–24.iii.2009: H0475 [QSBG]; 8–15.ix.2008: H0477 [QSBG]; ♂, 28.x–4.xi.2008: H0304 [QSBG]; 25.xi-2.xii.2008: H0310 [QSBG]; 28.iv–5.v.2009: H0332 [QSBG]; 21-28.iv.2009: H0592 [UKM]; 9–16.xii.2008: H0673 [HIC]; 18–25.xi.2008: H0675 [HIC]; 24-31.iii.2009: H0683 [HIC]; 31.viii–8.ix.2008: H0758 [HIC]; 9–16.vi.2009: H0972 [HIC], H0691 [HIC], H0666 [UKM]; ♂, Namtok Yong NP, 95m, 8.174°N, 99.742°E, MT, 9–16.ii.2009: H0351 [QSBG]; Namtok Mae Surin NP, Near Visitor Ctr, 228m, 19.358°N, 97.988°E, MT, 8–15.vii.2007: H5987 [UKM], H0617 [UKM], H0620 [UKM]; Namtok Mae Surin NP, 334m, 19.344°N, 97.988°E, MT, 26.viii–2.ix.2007: H5980 [HIC]; ♂, Namtok Yong NP, 80m, 8.174°N, 99.742°E, MT, 23–30.ix.2008: H0979 [HIC]; Mae Surin near visitor center, 228m, 19.489°N, 98.056°E, MT. 15–22.vii.2007: H0442 [QSBG], H0443 [QSBG]; ♀, Khao Pu-Khao Ya NP, 75m, 7.551°N, 99.789°E, MT, 28–30.v.2006: H5989 [UKM], H5995 [UKM]; 10–13.ii.2006: H5993 [UKM]; Tat Tone NP, 250m, 15.984°N, 102.035°E, litter, 23–26.vi.2006: H5997 [UKM]; Kaeng Krachan NP, km33/helipad, 735m, 12.836°N, 99.345°E, MT, 18–25.ii.2009: H0623 [UKM]; ♂, Doi Chiangdao NP, Pha Tang substation, 526m, 19.4163°N, 98.9147°E, MT, 3–10.xii.2007: H0926 [HIC].

Diagnosis.

Hind femur all pale; mesoscutum entirely pale; hind tibia mostly pale except apex black; stigma partly or entirely yellow; ocelli median ocellus distinctly narrower than space between lateral ocelli; lateral surface of hind femur densely punctate to aciculate, spaces between punctures not wider than diameter of punctures.

Description.

Body length 6.3 mm (6.0–6.4). Head. 40 flagellomeres. Median ocellus diameter narrower than space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate apically, veins yellow basally, melanic apically and around parastigma, stigma yellow in basal third. Hind tarsal claw bifid. Length/width of hind femur 1.027/0.519 = 3.7 (3.4–3.7). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 1.038/0.49 = 2.1 (2.0–2.1). Ratio of widest point of MT1 to narrowest point 0.49/0.313 = 1.6. Color. Yellow except as follows: antenna melanic, apex of hind tibia and entire tarsus melanic.

Figure 21.

Zelodia wichaii sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1–MT3.

Molecular data.

TaxaBank#/Genbank Accession: H063/JQ763453.

Distribution. Recorded from two localities in peninsular Thailand. Distribution map can be found at http://purl.org/thaimap/wichaii

Etymology.

Dedicated to Mr. Wichai Srisuka, the curator of insects at QSBG.

Material examined.

Holotype ♀. H0063 [QSBG], Thailand, Khoa Chong, Trang, 75m, 7.551°N, 99.789°E, MT, viii.2005.

Paratype. ♀, Thailand, Namtok Yon NP, Klong Jang Waterfall, 154m, 8.338°N, 99.827°E, MT, 12–19.v.2009: H0594 [HIC].

Diagnosis.

Hind tibia mostly pale except apex black; hind femur all melanic; mesoscutum entirely pale; median ocellus about as wide as or wider than space between lateral ocelli.

Description.

Body length 6.9 mm (male 5.8 – 6.7 mm). Head. 41 flagellomeres (41–43 males). Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli partly smooth, otherwise with weak punctures. Metapleuron sparsely covered with setae. Fore wing cells hyaline basally, weakly infuscate apically, veins yellow basally, except costa mostly melanic, melanic apically and around parastigma, stigma yellow in basal fifth. Hind tarsal claw bifid. Length/width of hind femur 2.28/.58 = 3.9 (3.45 – 3.52 males). Lateral surface of hind femur punctate. Metasoma. Length/width ratio of MT1, 1.22/.633 = 1.93. Ratio of widest point of MT1 to narrowest point .633/.385 = 1.6 (1.4–1.6). Color. Mostly yellow, black as follows: antenna except scape brown medially, hind leg except basal 4/5 of tibia yellow.

Figure 22.

Zelodia wirati sp.n. a lateral habitus b wings c dorsal head d lateral head e lateral mesosoma f dorsal propodeum g dorsal MT1–MT2.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H012/JQ763454; H949/ATRMK451-11/JQ763450.

Distribution.

Recorded from three localities in northwestern Thailand.Distribution map can be found at http://purl.org/thaimap/wirati

Etymology.

Dedicated to Mr. Wirat Sukho, collector for the TIGER project at Khao Yai National Park.

Material examined.

Holotype ♀.H0981 [QSBG], Thailand, Doi Phahompok NP, 569m, 19.966°N, 99.156°E, MT, 28.viii–4.ix.2007.

Paratypes. ♂ Thailand: Doi Inthanon NP, campground pond, 1200m, 18.544°N, 98.525°E, MT, 16–24.viii.2006: H0012 [QSBG]; Doi Chiangdao NP, Headquarters, 491m, 19.404°N, 98.9218°E, MT, 15–22.iv.2008: H0909 [HIC]; Chae Son NP, Doi Laan, 1528m, 18.825°N, 99.411°E, MT, 22–28.iii.2008: H949 [UKM].

Diagnosis.

Hind tibia all melanic; mesoscutum entirely pale; MT1 all white or cream colored, sometimes with a bit of melanic color subapically.

Description.

Body length 5.9 mm. Head. 35 (35–37) flagellomeres. Median ocellus diameter equal to the space between lateral ocelli. Vertex sparsely and weakly punctate. Mesosoma. Notauli mostly or entirely crenulate. Metapleuron densely covered with setae. Fore wing cells all weakly infuscate, veins all melanic. Hind tarsal claw bifid. Length/width of hind femur 1.714/0.475 = 3.5 (3.5–3.7). Lateral surface of hind femur aciculate. Metasoma. Length/width ratio of MT1, 3.978/0.350 = 2.8 (2.8–3.0). Ratio of widest point of MT1 to narrowest point 0.350/0.248 = 1.4. Color. Mostly black with orange, white, brown and cream color; head mostly orange, except area around ocelli slightly darker and antenna black; prothorax orange to brown; mesoscutum orange; scutellum brown, mesopleuron mostly black but orange-brown dorsally; fore leg yellow to cream colored; mid leg yellow to cream colored except coxa mostly melanic; MT1, M2 and part of MT3 cream colored (MT1 sometimes with a tan area posteromedially); anterior laterotergites and sterna white.

Figure 23.

Zelodia wirotei sp.n. a lateral habitus b wings c anterior head d lateral head e lateral mesosoma f dorsal mesosoma g dorsal propodeum and MT1–MT2.

Molecular data.

TaxaBank#/BOLD Process ID/Genbank Accession: H990/ATRMK272-11/JQ763451.

Distribution.

Recorded only from the type locality south-west of Bangkok. Distribution map can be found at http://purl.org/thaimap/wirotei

Etymology.

Dedicated to Mr. Wirote Naknan, chief of Namnao National Park.

Material examined.

Holotype ♀. H0634 [QSBG], Thailand, Kaeng Krachan NP, km33/helipad, 735m, 12.836°N, 99.345°E, MT, 11–18.v.2009.

Paratype. ♀, Thailand, Kaeng Krachan NP, Panernthung/km27, 950m, 12.822°N, 99.371°E, MT, 8–15.vi.2009: H0990 [HIC].

Acknowledgements

We thank all of the staff at Queen Sirikit Botanic Gardens in Chiang Mai for sorting the many hundreds of samples and for the Thai park staff for running Malaise traps and other collection devices. Thanks to Dr. van Achterberg for the loan of type specimens. A special thanks to Chaweewan Hutacharern for managing the Thai end of the TIGER project. Funding was provided by NSF grants DEB-0542864 and EF-0337220.

References
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Appendix I

DELTA data matrix, images, and other files to the dichotomous key for Zelodia (Hymenoptera: Braconidae: Agathidinae) from Thailand. doi: 10.3897/JHR.26.2527.app1

Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

Appendix 2

DELTA data matrix, images, and other files to species descriptions for Zelodia (Hymenoptera: Braconidae: Agathidinae) from Thailand. doi: 10.3897/JHR.26.2527.app2

Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

Appendix 3

Interactive key, in IntKey format, to Zelodia (Hymenoptera: Braconidae: Agathidinae) from Thailand. doi: 10.3897/JHR.26.2527.app3

Explanation note: To run the identification key, you will need Windows 95/NT or a later version.

You also need to download Intkey software and reboot your computer, if it is not already installed. The software package, Intkey, can be downloaded from http://delta-intkey.com/www/programs.htm. Once Intkey is installed you need only click on the .ink fi le (below) and the key will open. Click on any character on the left to begin.

More details on how to use Intkey efficiently are found at http://fl orabase.calm.wa.gov.au/help/keys/intkey_tutorial.pdf

Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

Appendix 4

Morphological terms matched to the Hymenoptera Anatomy Ontology. Identifiers (URIs) represent anatomical concepts in HAO version http://purl.obolibrary.org/obo/hao/2011-05-18/hao.owl

Abbreviations Term URI
LT3 abdominal laterotergite 3 http://purl.obolibrary.org/obo/HAO_0001456
MT1 abdominal mediotergite 1 http://purl.obolibrary.org/obo/HAO_0001463
MT2 abdominal mediotergite 2 http://purl.obolibrary.org/obo/HAO_0001150
MT3 abdominal mediotergite 3 http://purl.obolibrary.org/obo/HAO_0001147
antenna http://purl.obolibrary.org/obo/HAO_0000101
apical tarsomere http://purl.obolibrary.org/obo/HAO_0000994
area http://purl.obolibrary.org/obo/HAO_0000146
body http://purl.obolibrary.org/obo/HAO_0000182
carina http://purl.obolibrary.org/obo/HAO_0000188
costa http://purl.obolibrary.org/obo/HAO_0000225
coxa http://purl.obolibrary.org/obo/HAO_0000228
femur http://purl.obolibrary.org/obo/HAO_0000327
flagellomere http://purl.obolibrary.org/obo/HAO_0000342
flagellum, flagella http://purl.obolibrary.org/obo/HAO_0000343
fore leg http://purl.obolibrary.org/obo/HAO_0000349
fore wing http://purl.obolibrary.org/obo/HAO_0000351
frons http://purl.obolibrary.org/obo/HAO_0001044
head http://purl.obolibrary.org/obo/HAO_0000397
hind femur http://purl.obolibrary.org/obo/HAO_0001140
hind leg http://purl.obolibrary.org/obo/HAO_0000399
hind tarsus http://purl.obolibrary.org/obo/HAO_0001141
hind tibia http://purl.obolibrary.org/obo/HAO_0000631
lateral ocelli http://purl.obolibrary.org/obo/HAO_0000481
laterotergite http://purl.obolibrary.org/obo/HAO_0001861
leg http://purl.obolibrary.org/obo/HAO_0000494
median ocellus http://purl.obolibrary.org/obo/HAO_0000526
mediosternite http://purl.obolibrary.org/obo/HAO_0001654
S2 mediosternite 2 http://purl.obolibrary.org/obo/HAO_0001829
S3 mediosternite 3 http://purl.obolibrary.org/obo/HAO_0001830
mediotergite http://purl.obolibrary.org/obo/HAO_0001860
mesopleuron http://purl.obolibrary.org/obo/HAO_0000566
mesoscutum http://purl.obolibrary.org/obo/HAO_0001490
mesosoma http://purl.obolibrary.org/obo/HAO_0000576
mesothorax http://purl.obolibrary.org/obo/HAO_0000583
metapleuron http://purl.obolibrary.org/obo/HAO_0001869
metasoma http://purl.obolibrary.org/obo/HAO_0000626
metapectal-propodeal complex http://purl.obolibrary.org/obo/HAO_0000604
mid leg http://purl.obolibrary.org/obo/HAO_0000636
mouthparts http://purl.obolibrary.org/obo/HAO_0000639
notaulus http://purl.obolibrary.org/obo/HAO_0000647
ocellus http://purl.obolibrary.org/obo/HAO_0000661
ovipositor http://purl.obolibrary.org/obo/HAO_0001004
palpi http://purl.obolibrary.org/obo/HAO_0000683
pedicel http://purl.obolibrary.org/obo/HAO_0000706
propodeum http://purl.obolibrary.org/obo/HAO_0001249
prothorax http://purl.obolibrary.org/obo/HAO_0000874
puncture http://purl.obolibrary.org/obo/HAO_0000885
scape http://purl.obolibrary.org/obo/HAO_0000908
scutellar disc http://purl.obolibrary.org/obo/HAO_0000915
scutellar sulcus http://purl.obolibrary.org/obo/HAO_0000917
scutellum http://purl.obolibrary.org/obo/HAO_0000572
scutum http://purl.obolibrary.org/obo/HAO_0000575
metasomal segment http://purl.obolibrary.org/obo/HAO_0001969
seta http://purl.obolibrary.org/obo/HAO_0000935
sternite http://purl.obolibrary.org/obo/HAO_0000955
stigma http://purl.obolibrary.org/obo/HAO_0000957
tarsal claw http://purl.obolibrary.org/obo/HAO_0000989
tarsomere http://purl.obolibrary.org/obo/HAO_0000991
tarsus http://purl.obolibrary.org/obo/HAO_0000992
tendon http://purl.obolibrary.org/obo/HAO_0000996
terga http://purl.obolibrary.org/obo/HAO_0001006
tergite http://purl.obolibrary.org/obo/HAO_0001005
basal tooth http://purl.obolibrary.org/obo/HAO_0001219
trochantellus http://purl.obolibrary.org/obo/HAO_0001033
trochanter http://purl.obolibrary.org/obo/HAO_0001034
vein http://purl.obolibrary.org/obo/HAO_0001095
vertex http://purl.obolibrary.org/obo/HAO_0001077
wing http://purl.obolibrary.org/obo/HAO_0001089
wing cell http://purl.obolibrary.org/obo/HAO_0001091