Citation: Chavarría L, Noll FB (2014) Males of Neotropical social wasps (Vespidae, Polistinae, Epiponini) recognize colonies with virgin females. Journal of Hymenoptera Research 38: 135–139. doi: 10.3897/JHR.38.7763
Male behavior of Neotropical swarm-founding wasps has rarely been observed. The few published observations about male activities only describe their behavior during the short period they spend inside nests. In consequence, virtually nothing is known about what they do outside the colonies, and even less is known about mating behavior. This paper provides the first report of Epiponini males arriving at a colony with virgin females. The behavior of males and workers after queen removal was observed in one colony of Chartergellus communis located at a farm in Pedregulho, São Paulo, Brazil. The day after queen elimination, males were observed outside the nest. When males tried to enter the nest, workers aggressively attacked them. These attacks were similar to the defensive behavior used when foreign conspecifics attempt to enter the nest. The aggressive workers response and the absence of males before queen removal indicated that the males did not belong to the colony. Additionally, no fights were observed between individuals before queen removal. It is likely that foreign males arrived at the colony to mate with virgin females. Observations suggest that epiponine males are able to find nests with virgin females in mature stages of the colony cycle, and that mating can occur during different stages of the colony cycle.
Males, social wasps, queenless, colonies
Females are the foundation of Hymenoptera societies: queens are responsible for reproduction, and female workers perform all the tasks related to colony maintenance. Males do not play any essential social role in the colony, rather, they represent an energetic cost because they frequently request or rob food from workers (
Males of Epiponini wasps have been observed only during certain periods of the colony cycle: in the male production phase (
One colony of Chategergellus communis was observed for six months (05/2010 to 07/2010, 09/2010 to 11/2010), twice a week, four hours a day; at a farm located in Pedregulho, São Paulo State, Brazil (20°15'S, 047°27'W (DDM), 1041 m AMSL). A section of nest envelope was cut, and put back (as a door) every day, in order to perform the observations. To identify queens, some eggs were eliminated to stimulate oviposition. All queens and a sample of workers (N=40) were individually marked with quick-drying paint. During the first month of observations 11 females were observed laying eggs. Nevertheless the number of reproductive females decreased, until one remained as the sole egg layer in a colony of 298 adults. After five months of observations (24/10/10) the queen was eliminated in order to study the behavior of workers in an orphaned colony. Before queen removal the colony never passed through a male production stage, additionally no males were observed outside or inside the colony. After queen elimination males were observed flying outside the colony, their behavior and worker response to male presence were observed for three days after queen removal.
The day after queen elimination males were observed outside the colony. Males constantly tried to get into the nest (by the entrance and by the open door) inducing an aggressive response from workers. When workers perceived the presence of males, they immediately flew off the nest to face them and prevent their entrance. Workers fought aggressively against males during flight: they struck, bit, and grasped the male body. Sometimes fights were so violent that males and workers fell to the ground and continued fighting; one male was dead after an encounter with a worker. Before queen removal no aggressive behaviors were observed between colony members.
The workers’ response against males was similar to the defense response used when foreign individuals try to get access to colonies. Aggressive behaviors toward arriving males were also observed in a colony of Polybia liliacea in Brazil (Jeanne pers. comm) and in a colony of Vespa crabro (
Social wasps can recognize nestmates (
The observations of males arriving at the colony after queen elimination suggest males were attracted to the nest. It is possible that queen elimination stimulated some workers to develop their ovaries (
Our observations on Chartergellus suggest that mating strategies could involve the active searching for receptive females by males. Nevertheless, since epiponines show a great deal of variation in nest architecture, morphological caste syndromes, colony size, and distribution (
We especially thank Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (2009/07526-2; 2011/06058-5) for financial support; the Entomology graduate program of FFCLRP-Universidade de São Paulo, Brazil; Amauri and Edineia for allowing the performance of the observations in their farm in Pedregulho; and Sergio Jansen, Fabiana Fragoso and Sidnei Mateus for field assistance. We are grateful to Robert Jeanne for unpublished data and to Sergio Jansen and Robert Jeanne for their comments and suggestions essential for manuscript improvement. To Jack Neff and one anonymous reviewer for their suggestions and comments.