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Short Communication
First record of Megaphragma (Hymenoptera, Trichogrammatidae) in Columbia, and third animal species known to have anucleate neurons
expand article infoAlexey A. Polilov
‡ Lomonosov Moscow State University, Moscow, Russia
Open Access

Abstract

Megaphragma is recorded for the first time in Columbia where it is represented by M. caribea that emerged from leaves of Terminalia catappa infested by the thrips (Heliothrips haemorrhoidalis and Selenothrips rubrocinctus). M. caribea has anucleate neurons, the third species of Megaphragma shown to have this feature.

Keywords

Megaphragma caribea, anucleate neurons, Columbia

Introduction

Megaphragma includes some of the smallest insects. The genus contains 15 described species, distributed mainly in tropical and subtropical regions; only five of them have been recorded in the Western Hemisphere (Pinto 2006, Viggiani et al. 2009). A unique feature has been described in two of the species: the cell bodies and nuclei undergo lysis during a later pupal stage in over 95% of the neurons. As a result, the central nervous system of the adult is represented mostly by anucleate neurons (Polilov 2012). M. caribea Delvare, 1993, known only from the type series collected in Guadeloupe, is the smallest species of Megaphragma with a body length reported as 0.17 mm (Delvare 1993) and one of the smallest flying insects (Huber and Noyes 2013). Further information on the biology and distribution of M. caribea is reported here.

Methods

Adults of M. caribea (27 specimens) were collected as they emerged from leaves of Terminalia catappa Linneaus, 1767 colonized by the thrips Heliothrips haemorrhoidalis (Bouché, 1833) and Selenothrips rubrocinctus (Giard, 1901). The leaves were gathered in Cartagena, Columbia, on 22 January 2015 (coordinates 10.422, -75.553).

Specimens were fixed in FAE (formaldehyde—acetic acid—ethanol), preserved in 70% ethanol, critical point dried (Hitachi HCP-2), sputter coated with gold (Giko IB-3) and examined under a Jeol JSM-6380 scanning electron microscope (SEM) where body length was measured. For studying internal morphology, fixed material was dehydrated and embedded in Araldite M. The resulting blocks were cut into complete series of cross sections or longitudinal sections 0.5 μm thick using a Leica RM2255 microtome. The sections were stained with DAPI and studied under an Olympus BX43 microscope with a fluorescent module and a Tucsen TCC-6.1ICE camera.

Results and discussion

The record of M. caribea in Columbia considerably expands the known range of this species known previously only from 11 specimens collected from leaves of Psidium guajava infested by Selenothrips rubrocinctus in Vieux Habitans (Guadeloupe) by J. Etienne on 17 November 1988 (Delvare 1993). The host plant and host insect(s) recorded here increase the range of plants and possibly host Thysanoptera in which M. caribea can develop.

M. caribea differs from the other species of the genus in its smaller body size, antenna without funicle, two-segmented antennal club, and wing chaetotaxy (Figs 1, 2). The body length of M. caribea measures was 181–224 μm (M = 199, n = 12), which is slightly greater than the measurement (170 μm) provided by Delvare (1993). Similar imprecision of measurements was recently shown also for the smallest known free-living insect, the beetle (Polilov 2015).

Figure 1. 

Habitus of Megaphragma caribea, SEM: A dorsal view B lateral view.

Figure 2. 

Diagnostic characters of Megaphragma caribea, SEM: A head of female B head of male C antennal club, male D antennal club, female E wings F terga of metasoma.

Analysis of the anatomy of M. caribea has shown that the central nervous system of this species has only a few nuclei (Fig. 3) instead of the cortical layer typical of other insects. The central nervous system of M. caribea contains about 600 nuclei, 390 of them in the cerebrum. Organization of the neuropil is otherwise no different from that of other hymenopterans. Taking into account the relative volume and structure of the neuropil and the number of nuclei, which is similar to other species of Megaphragma examined earlier but fundamentally different from that of other minute insects. I suggest that M. caribea also displays the unique phenomenon of lysis of cell bodies and nuclei in neurons prior to the emergence of the adult from the pupa, as described earlier in M. mymaripenne (Polilov 2012) and M. amalphitanum (Polilov 2017). Thus, all species of Megaphragma whose anatomy has been studied have unique anucleate neurons. Interestingly, the nervous system of M. caribea contains almost twice as many nuclei as in the larger representatives of the genus.

Figure 3. 

Internal morphology of Megaphragma caribea: A scheme of sections, dorsal view B–F sagittal sections, DAPI and autofluorescence; ag – abdominal ganglion, cer – cerebrum, gg1,2,3 – pro-, meso-, and metathoracic ganglion.

Acknowledgements

The study of nervous system was supported by the Russian Foundation for Basic Research (project nos. 17-04-00669); the rest of this study was supported by the Russian Science Foundation (project no. 14-14-00208).

References

  • Delvare G (1993) Sur les Megaphragma de Guadeloupe avec la description d‘une espèce nouvelle (Hymenoptera, Trichogrammatidae). Revue Française d‘Entomologie (nouvelle série) 15(4): 149–151.
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