Research Article |
Corresponding author: Yan-Zhou Zhang ( zhangyz@ioz.ac.cn ) Academic editor: Petr Janšta
© 2018 Guo-Hao Zu, Xu Zhang, Cheng-De Li, Yan-Zhou Zhang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zu G-H, Zhang X, Li C-D, Zhang Y-Z (2018) A new species of Anagyrus (Hymenoptera, Encyrtidae) from Malaysia, parasitoid of Lanceacoccus sp. (Hemiptera, Pseudococcidae). Journal of Hymenoptera Research 65: 141-148. https://doi.org/10.3897/jhr.65.24872
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A new species of Anagyrus Howard, 1896 (Hymenoptera: Encyrtidae), A. minipedicellus Zu & Zhang sp. n., is described from Borneo of Malaysia as a parasitoid of Lanceacoccus sp. (Hemiptera: Pseudococcidae), and DNA barcoding of the new species is given.
Chalcidoidea , Tetracneminae , Anagyrini , new species, Sabah
Anagyrus Howard, 1896 is one of the most diverse genus of Encyrtidae, containing 282 species worldwide (
The genus has got comparatively little attention in Malaysia.
In the present paper, A. minipedicellus Zu & Zhang, sp. n. is described as new to science, and a key to Malaysian species of Anagyrus is provided.
All the specimens in the present study were collected from Mt. Trus Madi by rearing, then dissected and mounted in Canada balsam on slides following the method described by
Eight individuals of A. minipedicellus were used to obtain their DNA barcodes. Total genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s protocols. Polymerase chain reactions (PCR) were carried out in 50 μL reaction volumes using TaKaRa ExTaq Polymerase kit. Final volumes included 5 μL of 10×Buffer, 25 mM MgCl2, 2.5 mM dNTP mixture, 10 pmol of each primer, 1U of ExTaq and 5 μL of genomic DNA. The COI gene fragment was amplified using the primers FWPTF1 (
The following abbreviations are used in the text:
F1–6 funicular segments 1–6
AOL minimum distance between a posterior ocellus and anterior ocellus
OCL minimum distance between a posterior ocellus and occipital margin
OD longest diameter of an ocellus
OOL minimum distance between a posterior ocellus and eye margin
POL minimum distance between posterior ocelli
MT length of mid tibia
OL length of the ovipositor
1 | Fore wing infuscate in apical 2/3 | A. thailandicus |
– | Fore wing hyaline | 2 |
2 | Head unicolorous, dark brown or black | 3 |
– | Head at least partially orange | 5 |
3 | Antenna with F1–F5 dark brown | A. tricolor |
– | Antenna with at least F4 and F5 yellow or white | 4 |
4 | Ovipositor clearly exserted, the exserted part at least about 2/3 length of mid tibial spur; occipital margin behind posterior ocelli with a sharp raised carina | A. malayensis |
– | Ovipositor not or hardly exserted; occipital margin behind posterior ocelli sharp but without a raised carina | A. luci |
5 | Only F1 dark brown, remaining segments of funicle white | 6 |
– | Funicle with at least F1–F3 completely or partially dark brown | 7 |
6 | Body quite flattened and elongate; fore wing nearly 2.5× as long as broad; ovipositor about 1.5× as long as mid tibia | A. saccharicola |
– | Body stout, not elongate and not dorsoventrally flattened; fore wing about 2× as long as broad; ovipositor slightly shorter than mid tibia | A. chrysos |
7 | F1 clearly longer than pedicel; F6 brown | A. minipedicellus sp. n. |
– | F1 at least slightly shorter than pedicel; F6 yellow or white | 8 |
8 | Ovipositor about 0.69× as long as mid tibia; F1 much shorter than any other funicular segments | A. subtilis |
– | Ovipositor about 1.17× as long as mid tibia; F1 similar to others | A. ephyra |
♀ [on slide], MALAYSIA, Sabah, Mt. Trus Madi, 5°26'27"N; 116°27'0"W, c. 1180m, 8.II.2017, Guo-Hao Zu, rearing from Lanceacoccus sp. (Hemiptera: Pseudococcidae) (Fig.
4♀, 4♂ [4♀, 2♂ on slides], the same data as holotype (
Female. Length, excluding ovipositor, 1.38–1.83 mm. Body stout (Fig.
Male (length, 1.22–1.33 mm): Color similar to female (Fig.
Female. Holotype. Length, 1.83 mm (excluding ovipositor). Head generally orange, interantennal prominence dark brown, gena dark brown to mid eye level, occiput mostly dark brown. Antenna with radicle dark brown; scape dark brown with a white subapical band; pedicel and funicle black, funicle gradually becoming paler distad and ventrally paler, clava white. Mesosoma dark brown; mesopleuron orange. Wings hyaline. Legs generally yellowish white, except dorsal and ventral margins of fore coxa, fore and mid femora, hind tibia dark brown, mid coxa mostly dark brown, hind coxa brown, all tarsi yellowish brown. Metasoma dark brown.
Head. Frontovertex 0.37× head width, with very fine scale-like sculpture; setae on frontovertex about half as long as diameter of anterior ocellus; ocelli forming an angle of about 85°; posterior ocellus slightly closer to occipital margin than to eye margin; eye reaching occipital margin, with dense, dark brown setae, each seta about 1.5× as long as a facet width; head, in frontal view (Fig.
Mesosoma (Fig.
Metasoma 0.86× as long as mesosoma; ovipositor (Fig.
Length of female, excluding ovipositor, varies from 1.38–1.83 mm, scape in smaller species as in Fig.
Male. Length 1.22–1.33 mm. Color is similar to that of female except head completely black, flagellum gradually going from dark brown to yellowish. Antenna (Fig.
Lanceacoccus sp. (Hemiptera: Pseudococcidae).
The specific name refers to the small pedicel of female antennae.
This species is similar to A. alami Hayat, 1970 in the short and narrow pedicel. However, it can be easily separated from A. alami by its dark brown body (generally yellowish in alami), F1 1.33× as long as pedicel (twice in A. alami), MT 1.42× as long as OL (1.05× in borneensis).
It is also probably close to A. subtilis Noyes & Hayat, 1994 and A. aceris Noyes & Hayat, 1994, but differs in the short pedicel, which is 0.75× as long as F1 (at least longer than F1 in A. subtilis and A. aceris), normal F1, which is similar to other funicular segments (F1 much shorter than any other funicular segments in A. subtilis), dark brown radicle (orange in A. subtilis), subparallel eye margins (diverging ventrally in A. aceris) and generally orange mesosoma (dorsum of mesosoma dark brown in A. aceris).
The COI sequences the eight individuals of A. minipedicellus were successfully generated with high quality. All sequences have been deposited in GenBank (Accession Numbers: MH587108–MH587115). Only one base pair changes was detected between the eight individuals sequenced for COI. Blasting the COI sequences gave no close matches (over 90%) both on BOLD and GenBank. In GenBank, a COI sequence of Anagyrus (Anagyrus nr. pseudococci PLCO02, Accession No. KU499515) differs from those of the new species by about 14%.
This project was supported by the Scientific Project of Tianjin Municipal Education Commission (No. 2017KJ185). We are grateful to Dr. Xu-Bo Wang (Beijing Forestry University) for the identification of host species.