Research Article |
Corresponding author: Sergio R. Sánchez-Peña ( sanchezcheco@gmail.com ) Academic editor: Jose Fernandez-Triana
© 2019 Moisés Felipe-Victoriano, Elijah J. Talamas, Sergio R. Sánchez-Peña.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Felipe-Victoriano M, Talamas EJ, Sánchez-Peña SR (2019) Scelionidae (Hymenoptera) parasitizing eggs of Bagrada hilaris (Hemiptera, Pentatomidae) in Mexico. In: Talamas E (Eds) Advances in the Systematics of Platygastroidea II. Journal of Hymenoptera Research 73: 143-152. https://doi.org/10.3897/jhr.73.36654
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The painted bug or bagrada bug, Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae), is a key pest of crops in the family Brassicaceae. In this work, three species of Scelionidae (Hymenoptera) are reported for the first time as parasitoids of painted bug eggs in Mexico, at Saltillo, state of Coahuila: Gryon myrmecophilum (Ashmead), Telenomus podisi Ashmead and Trissolcus basalis (Wollaston). This is also the first report of a species of the widespread genus Telenomus as an egg parasitoid of B. hilaris outside of India. Total percent parasitism, high resolution images, and CO1 sequences are provided for each species. In the future, research in Mexico should be carried out on parasitoid species presented in this work to determine their potential as biological control agents and the feasibility of augmentative, classical or inoculative biocontrol strategies for integrated pest management.
Heteroptera, stink bug, biological control, parasitoid
Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae), known in Mexico with the common names of bagrada bug or painted bug, is a key pest of cole crops (family Brassicaceae) originally distributed in Africa and Asia (
The family Scelionidae is a cosmopolitan group of parasitoids that attacks the eggs of a variety of arthropods, including Hemiptera. In the Old World, several authors have reported parasitoids of this family attacking painted bug eggs. In India, Gryon karnalense (Chacko and Katiyar) and Telenomus samueli Mani were reported from the eggs of B. hilaris (as Bagrada cruciferarum Kirkaldy) (
The work was carried out in the experimental fields of the Universidad Autónoma Agraria Antonio Narro (
Eggs were obtained by rearing field-collected mating pairs of painted bugs in the laboratory. Eight mating pairs were placed in Petri dishes at a temperature of 26–28 °C with diffuse overhead daylight. After 12 hours in the laboratory, mating pairs in the Petri dishes produced an average of 270 eggs (range of 85–580). The mating pairs were removed and the eggs (on the same uncovered Petri dish bottom they were laid on) were placed on the soil surface at a distance of approximately 5 cm from a broccoli stem. If it was necessary to handle the eggs, a soft number 2 brush (Pinceles Rex, Mexico City) was used.
The sentinel egg tests were conducted monthly in the field from 25 November 2017 – 20 December 2018. The eggs were exposed 7–8 days in the field, and subsequently incubated at 24–28 °C and a relative humidity of 60% in the laboratory until the emergence of parasitoids. The wasps that emerged were placed in 96% ethanol until their subsequent identification.
Specimens were softened in 70% ethanol for two hours, then DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen). DNA extracts were quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific). At least 20 ng of genomic DNA was used per PCR. The 5’-CO1 barcode region was PCR-amplified using the primers LCO1490 and HCO2198 (
Species | Locality | Collection Unit Identifier | GenBank Accession Number |
---|---|---|---|
Trissolcus basalis | Saltillo, Mexico |
|
MK720829 |
Telenomus podisi | Saltillo, Mexico |
|
MK720830 |
Gryon myrmecophilum | Saltillo, Mexico |
|
MK720831 |
Gryon myrmecophilum | Saltillo, Mexico |
|
MK720832 |
Gryon myrmecophilum | Rutgers, NJ, USA |
|
MK937524 |
Specimens of G. myrmecophilum and Te. podisi were identified to species using the keys of
Stacks of photographs were taken with a Macropod imaging system and rendered using HeliconFocus. Specimen collection data and host associations are deposited in the Hymenoptera Online Database (htttp://hol.osu.edu). Voucher specimens for all scelionid species are deposited at the Florida State Collection of Arthropods (
Scelionid wasps were detected only in November 2017 and June, July and August 2018. In a subsequent paper we will discuss in detail the phenology of the parasitoid complex on painted bug eggs at this location in Mexico.
Figs
As reported by
BLAST comparison of the CO1 sequence from specimen
On the collection date of 25 November 2017, a total of 29 Tr. basalis were collected (this date was the only time Tr. basalis emerged from sentinel eggs) and the percentage of parasitism was 12.4% (n= 234 eggs).
Figs
The small size of B. hilaris eggs does not influence the diagnostic morphology of Te. podisi and no relevant differences were found between the specimens in this study and Te. podisi reared from other stink bug eggs. BLAST comparison of the CO1 sequence from specimen
On the monthly collection dates between January-December 2018, a total of 51 Te. podisi was collected in the months of June and July, resulting in 9.2 and 9.9% of parasitism respectively (n= 532 eggs).
Figs
Our identification of this species is based on the revision of the genus by
On the monthly collection dates between January–December 2018, a total of 115 G. myrmecophilum were collected in the months of June, July and August, resulting in 3.0, 7.6 and 43.2% of parasitism respectively (n=786 eggs).
In 2017, only Tr. basalis emerged from eggs, in the month of November (29 specimens), for 12.4% of parasitism. This wasp is a near-cosmopolitan parasitoid of stink bug eggs for which one widespread host is the southern green stink bug, Nezara viridula (L.) (
During the 2018 monthly sampling dates, scelionid wasps were detected only in June–August. The percentages of egg parasitism by all Scelionidae in the months of June, July and August 2018 correspond to 12.2, 17.4 and 49.6% respectively (total of 166 wasp specimens). Out of the total monthly percent parasitism, Gryon myrmecophilum contributed with 24.2, 43.5 and 100% in June, July and August, respectively (115 specimens total); and Te. podisi contributed with 75.8 and 56.5%, for the months of June and July, respectively (51 specimens total). Species of Telenomus have been reported as a parasitoids of other stink bugs, including Euschistus heros (F.), Halyomorpha halys (Stål), Oebalus insularis Stål, Piezodorus guildinii (Westwood) and Tibraca limbativentris (Stål).
In Pakistan,
To our knowledge, the present work reports the highest percentage of field parasitism of painted bug eggs in the USA and Mexico. It is also the first report of Te. podisi parasitizing painted bug eggs. We continue studying the identity, distribution and population fluctuation of beneficial wasps associated with painted bug eggs at selected localities in Mexico. Future research should be carried out on these species, and possibly others that have yet to be detected, to determine their potential as biological control agents. In particular, there is a need for critical comparative analysis of the different modalities of biological control (classical, augmentative or inoculative) that can utilize parasitic wasps in the integrated pest management of the painted bug in Mexico.
This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) scholarship 300868 to MFV; Dirección de Investigación,