Research Article |
Corresponding author: Sanna Keronen ( thunderwaves@gmail.com ) Academic editor: Gavin Broad
© 2021 Sanna Keronen, Ilari E. Sääksjärvi, Tapani Hopkins.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Keronen S, Sääksjärvi IE, Hopkins T (2021) Deducing how tropical rhyssines (Hymenoptera, Ichneumonidae) mate from body measurements. Journal of Hymenoptera Research 86: 93-100. https://doi.org/10.3897/jhr.86.71615
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The biology of many Darwin wasp (Hymenoptera: Ichneumonidae) species is poorly known. Existing museum specimens can potentially be used to get information on e.g. how species live, what they eat, and what their life cycle is. One example of this is a 1991 study by Eggleton in which he measured some rhyssine (Ichneumonidae: Rhyssinae) species, and used the results to deduce how the species likely mate. We extend this work by measuring five tropical species. We found no evidence that the males of our species scramble for females before the females emerge, which matches what was hypothesised by Eggleton. Further measurements of more species would provide information on how other species mate, and field observations of mating rhyssines would help confirm that Eggleton’s method for deducing rhyssine mating strategies gives true results.
Amazon Malaise trapping 2000, Darwin wasps, Ichneumonidae, Rhyssinae, Uganda Malaise trapping 2014–2015
The Darwin wasps (Hymenoptera: Ichneumonidae) are an extremely diverse but poorly known family, with possibly over 100000 species (
Existing museum specimens can be used to deduce details of the biology of a species.
Eggleton then suggested using this information to deduce the likely mating strategy of other species. Eggleton’s sample sizes were very small, however, so he was unable to deduce the mating strategy of more than a few (mainly European or North American) species. Five of his species were from the genus Epirhyssa Cresson, 1865, a mainly tropical genus found in both the Old and New World, and one that is suspected not to be monophyletic (
After
Our material consisted of 107 male rhyssines in 5 species. Three of these species were collected in Uganda: Epirhyssa ghesquierei Seyrig, 1937 (42 males), E. overlaeti Seyrig, 1937 (16 males) and E. quagga
To find out which mating strategy these species most likely use, we measured the length of the male mesosoma and the slenderness (length/width) of the third metasomal tergite. The former was used as a proxy for body size, and the latter as a proxy for metasoma slenderness. We measured the mesosoma from the foremost edge of the mesoscutum/pronotum to the insertion of the metasoma (Figure
Measurements taken. We estimated the size of a rhyssine wasp by measuring the length of the mesosoma (A). We estimated the slenderness of the metasoma by measuring the length (B) and width (C) of tergite 3. The forewing (D) of some wasps was also measured. Note that the left forewing is blurred in this image; these measurement images were taken mainly for the purpose of future reference, so details are not always as clear as during the measurement itself.
We plotted metasoma slenderness versus body size, fitted a linear regression line to the plot, and interpreted a significantly increasing line (slenderness increase in large males) as evidence of the BESC mating strategy. For consistency with
We analysed the data in the R software v. 3.4.0 (
Our analyses differ somewhat from those of
Eggleton also used correlations, instead of linear regression, to analyse his data; again, we expect the results to be similar whichever method is used. The p-values in particular are calculated the same way, since both are based on the t-statistic; the only difference arises from Eggleton’s analyses using a one-tailed test whereas the linear regressions are two-tailed. We felt that a linear regression with its corresponding plots gives a better visualisation of the results, but give the p-values of both approaches.
Mesosoma length matched the previously used measure of body size, wing length (linear regression p < 0.01, R2 = 0.81; Figure
The slenderness of tergite 3 did not significantly vary with mesosoma length in any of our study species (linear regression, p range = 0.09–0.44, R2 range = 0.03–0.19, Figures
Mesosoma length and tergite 3 slenderness (length/width) of 107 male rhyssine wasps. Males of all species had similarly slender tergites 3, irrespective of body size. The only exception was Epirhyssa overlaeti, whose tergites got significantly more slender with body size when the significance was estimated by one-tailed correlation (c.f. Figure
Mesosoma length versus tergite 3 slenderness (length/width) of 107 male rhyssine wasps. No species showed a significant increase in slenderness with body size (linear regression, statistics in top margin). This suggests the males of these species do not scramble for emerging females. The one exception was Epirhyssa overlaeti, whose tergites got significantly more slender with body size when the significance was estimated by one-tailed correlation (p-values marked with †) instead of linear regression.
We found (almost) no evidence that the males of our tropical study species scramble for females before the females emerge. The one exception was Epirhyssa overlaeti which showed a significant increase in tergite slenderness with body size, when analysed with one-tailed correlation tests instead of linear regression. We do not feel this merits too much attention, since this was only marginally significant and we would in any case expect some false positives when testing multiple species.
These results fit what has been hypothesised.
Although our results provide some interesting insights on the biology of some rhyssine species, it remains a fact that there are over 250 rhyssine species (