Research Article |
Corresponding author: Elijah Talamas ( billy.jenkins@GMAIL.COM ) Academic editor: Miles Zhang
© 2022 Cheng-Jin Yan, Elijah Talamas, Zachary Lahey, Hua-Yan Chen.
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Yan C-J, Talamas E, Lahey Z, Chen H-Y (2022) Protelenomus Kieffer is a derived lineage of Trissolcus Ashmead (Hymenoptera, Scelionidae), with comments on the evolution of phoresy in Scelionidae. Journal of Hymenoptera Research 94: 121-137. https://doi.org/10.3897/jhr.94.95961
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Species of the genus Protelenomus Kieffer (Platygastroidea, Scelionidae) are phoretic egg parasitoids of coreid bugs. The discovery, DNA sequencing, and molecular phylogenetic analysis of a Protelenomus species phoretic on Cletus punctiger (Dallas) (Hemiptera, Coreidae) shows that Protelenomus is a derived lineage of Trissolcus Ashmead. Protelenomus is treated as a junior synonym and a new species of phoretic Trissolcus, T. siliangae Yan, Chen & Talamas, is described from China.
Coreidae, egg parasitoid, Hemiptera, integrated taxonomy, phylogenetics
Scelionid parasitoids of hemipteran eggs are the subject of active study, driven by the economic damage caused by a variety of bug pests, and by recent works that accelerate further advancement. These include progress in the taxonomy and systematics of these parasitoids, which underlie accurate identification and classification (
Even modification of the legs, which is pronounced in some species (e.g., P. tibialis Veenakumari), is not ubiquitous in Protelenomus. Given the nebulous boundary between Protelenomus and Trissolcus, we investigated the possibility that Protelenomus is a lineage derived from within Trissolcus, an idea first proposed by
During a survey of insect pests and their parasitoids in a corn field in Wenzhou, Zhejiang Province, China, in the autumn of 2021, four female coreid bugs were found to each harbor a phoretic wasp dorsally on the head near the base of the antennae Fig.
Abbreviations and morphological terms used in text: A1, A2, ... A12: antennomere 1, 2, … 12; OOL: ocellar-ocular length; POL: posterior ocellar length; OD: ocellar diameter; T1, T2, ... T7: metasomal tergite 1, 2, ... 7; S1, S2, ... S7: metasomal sternite 1, 2, … 7. Morphological terminology otherwise generally follows
sasu subacropleural sulcus (Fig.
mtnm metanotum (Figs
mtpm metapostnotum (Figs
pl parapsidal line (Fig.
ppm propodeum (Figs
Genomic DNA was extracted using a TIANamp Micro DNA Kit (Tiangen Biotech (Beijing), Co.., Ltd), following the nondestructive DNA extraction protocol described in Taekul et al. (2014). Four molecular markers were amplified: two nuclear ribosomal (18S and 28S D2-3), one mitochondrial protein (COI), and one single-copy nuclear protein (wingless). Polymerase chain reactions were performed using Tks Gflex DNA Polymerase (Takara) with primer pairs shown in Table
Gene | Primer name | Primer sequence 5’ to 3’ | Reference |
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18S | ai | CCTGAGAAACGGCTACCACATC |
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18S | 18S-5R | CTTGGCAAATGCTTTCGC |
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28S | D23F | GAGAGTTCAAGAGTACGTG |
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28S | 28Sb | TCGGAAGGAACCAGCTACTA |
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COI | HCO-2198 | TAAACTTCAGGGTGACCAAAAAATCA |
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COI | LCO-1490 | GGTCAACAAATCATAAAGATATTGG |
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Wingless | SceWgIF-1 | GTAAGTGTCACGGGATGTC |
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Wingless | SceWgIR-1 | TTGACTTCACAGCACCAGT |
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Multiple sequence alignments for each gene were performed with MAFFT v7.490 (
Photographs of live specimens were taken with a Canon 5D Mark III (Tokyo, Japan) camera with a 100 mm macro lens. Multifocal images of mounted specimens were made using a Nikon SMZ25 microscope with a Nikon DS-Ri 2 digital camera system and a Macropod Microkit photography system. All image stacks were rendered using Helicon Focus. Scanning electron micrographs were produced using a Phenom Pro Desktop SEM. Images were post-processed with Adobe Photoshop CS6 Extended.
The phylogenetic analysis retrieved Trissolcus siliangae Yan, Chen & Talamas sp. nov. embedded within Trissolcus, as the sister taxon to a clade comprising (T. vindicius + T. cultratus) + (T. corai + (T. japonicus + T. plautiae)) (Fig.
Treatment of Protelenomus as a derived lineage of Trissolcus is also supported by a morphological character, the subacropleural sulcus.
Trissolcus Ashmead
Trissolcus Ashmead, 1893: 161 (original description. Type: Telenomus brochymenae Ashmead, by original designation. Key to species).
Asolcus Nakagawa, 1900: 17 (original description. Type: Asolcus nigripedius Nakagawa, by monotypy. Synonymized with Trissolcus by
Protelenomus Kieffer syn. nov., 1906: 6 (original description. Type: Protelenomus flavicornis Kieffer, by monotypy).
Aphanurus Kieffer, 1912: 10, 69 (original description. Type: Teleas semistriatus Nees von Esenbeck, by original designation. Preoccupied by Aphanurus Looss (1907) (Trematoda).
Immsia Cameron, 1912: 104 (original description. Type: Immsia carinifrons Cameron, by monotypy. Synonymized with Microphanurus Kieffer by
Microphanurus Kieffer:
Epinomus Ghesquière, 1948: 324 (original description. Type: Epinomus anoplocnemidis Ghesquière, by monotypy and original designation. Synonymized with Protelenomus by
Latonius Kononova, 1982: 76 (original description. Type: Latonius planus Kononova, by monotypy and original designation. Synonymized with Trissolcus by
Kozlotelenomus Mineo, O’Connor & Ashe, 2009: 193 (original description. Type: mopsus Nixon, by monotypy and original designation. Synonymized with Trissolcus by Talamas and Buffington (2015)).
Ioseppinella Mineo, O’Connor & Ashe, 2010: 267 (original description. Type species Ioseppinella serena Mineo, O’Connor & Ashe, by monotypy and original designation. Synonymized with Trissolcus by
Trissolcus anoplocnemidis (Ghesquière), comb. nov.
Epinomus anoplocnemidis Ghesquière, 1948: 325 (original description);
Protelenomus anoplocnemidis (Ghesquière):
Trissolcus areolatus (Rajmohana), comb. nov.
Protelenomus areolatus Rajmohana, 2013: 2 (original description, diagnosis);
Trissolcus flavicornis (Kieffer), comb. nov.
Protelenomus flavicornis Kieffer, 1906: 7 (original description);
Trissolcus gajadanta (Veenakumari), comb. nov.
Protelenomus gajadanta Veenakumari, 2019: 384 (original description, keyed)
Trissolcus lutuli (Veenakumari), comb. nov.
Protelenomus lutuli Veenakumari, 2019: 384, 385 (original description, keyed)
Trissolcus maasai (Veenakumari), comb. nov.
Protelenomus maasai Veenakumari, 2019: 384, 386 (original description, keyed)
Trissolcus tibialis (Veenakumari), comb. nov.
Protelenomus tibialis Veenakumari, 2019: 384, 387 (original description, keyed)
Trissolcus yao (Veenakumari), comb. nov.
Protelenomus yao Veenakumari, 2019: 384, 388 (original description, keyed)
Trissolcus zulu (Veenakumari), comb. nov.
Protelenomus zulu Veenakumari, 2019: 384, 388 (original description, keyed)
Female body length: 1.28 mm (n = 4). Body color: head, mesosoma, and metasoma black, shining. Mandible color: red-brown. Leg color: coxae and tarsi dark brown, rest of legs yellow-brown. Tegulae yellow-brown. Antennal color: radicle and A1–A2 yellow to brown, darker dorsally; A3–A11 dark brown.
Head. Length of radicle: less than width of clypeus. Claval formula: A8–A11:1-1-1-1. Facial striae: present. Number of clypeal setae: 4. Shape of gena in lateral view: moderately wide, bulging. Genal carina: absent. Malar striae: absent. Sculpture of malar sulcus: smooth. Orbital furrow: expanded at intersection with malar sulcus, medial margin of furrow poorly defined. Macrosculpture of frons directly dorsal to the antennal scrobe: absent. Preocellar pit: absent. Setation of lateral frons: sparse. Punctation of lateral frons: absent. Sculpture directly ventral to preocellar pit: coriaceous microsculpture. Rugae on lateral frons: absent. OOL: about one ocellar diameter. Hyperoccipital carina: absent. Macrosculpture of posterior vertex: absent. Microsculpture on posterior vertex along occipital carina: coriaceous. Anterior margin of occipital carina: crenulate. Medial part of occipital carina in dorsal view: rounded.
Mesosoma. Epomial carina: present. Macrosculpture of lateral pronotum directly anterior to netrion: finely rugulose. Netrion sulcus: incomplete, only weakly defined ventrally. Pronotal suprahumeral sulcus in posterior half of pronotum: absent. Number of episternal foveae: 2. Course of episternal foveae ventrally: abutting dorsal apex of acetabular carina. Course of episternal foveae dorsally: distinctly separate from mesopleural pit. Subacropleural sulcus: present. Speculum: transversely strigose. Mesopleural pit: simple. Mesopleural carina: absent. Sculpture of femoral depression: smooth. Patch of striae at posteroventral end of femoral depression: present, striae orthogonal to long axis of femoral depression. Setal patch at posteroventral end of femoral depression: present as a line of setae. Microsculpture of anteroventral mesopleuron: present in anterior portion, smooth posteriorly. Macrosculpture of anteroventral mesopleuron: absent. Postacetabular sulcus: present as a smooth furrow. Mesopleural epicoxal sulcus: indicated by shallow foveae. Setation of posteroventral metapleuron: absent. Sculpture of dorsal metapleural area: rugulose. Posterodorsal metapleural sulcus: undifferentiated. Paracoxal sulcus in ventral half of metapleuron: absent. Length of anteroventral extension of metapleuron: short, not reaching base of mesocoxa. Metapleural epicoxal sulcus: indistinguishable from rugose sculpture. Mesoscutal humeral sulcus: comprised of shallow foveae. Median mesoscutal carina: absent. Microsculpture of mesoscutum: coriaceous. Mesoscutal suprahumeral sulcus: comprised of shallow foveae. Length of mesoscutal suprahumeral sulcus: about two-thirds the length of anterolateral edge of mesoscutum. Parapsidal line: present. Notaulus: absent. Median protuberance on anterior margin of mesoscutellum: absent. Shape of dorsal margin of anterior lobe of axillar crescent: flat. Sculpture of anterior lobe of axillar crescent: dorsoventrally strigose. Area bound by axillar crescent: smooth. Macrosculpture of mesoscutellum: absent. Microsculpture on mesoscutellum: coriaceous. Median mesoscutellar carina: absent. Setation of posterior scutellar sulcus: absent. Form of metascutellum: broad, short, rugose projection. Metanotal trough: foveate, foveae occupying less than half of metanotal height. Metapostnotum: invaginated laterally, propodeum and metanotum directly adjacent. Anteromedial portion of metasomal depression: rugulose.
Wings. Length of postmarginal vein: about twice as long as stigmal vein. Fore wing apex: reaching beyond T6.
Legs. Color: coxae and distal tarsomeres dark brown to black, otherwise yellow to light brown. Anteroventral area of hind femora: not covered by setae. Femur and tibia not enlarged. Basitarsi of fore leg with a row of densely stout bristles at basal half. Claws well developed, curved.
Metasoma. Width of metasoma: about equal to width of mesosoma. Longitudinal striae on T1 posterior to basal costae: present. Number of sublateral setae (on one side): 0. Setation of laterotergite 1: absent. Striation on T2: extending about half the length of the tergite, weakly indicated. Setation of T2: present along lateral margin. Setation of laterotergite 2: present. Striation on S2 striate: present laterally, length of striae extending up to anterior half, remainder smooth. S2 felt fields: present. Sculpture of S3–S6: setigerous punctate.
Male. Unknown.
Moniliform antennae in females are rare in Scelionidae, shared in Trissolcus by T. siliangae, T. flavicornis, T. gajadanta, and T. planus; these species also have a single papillary sensillum on each clavomere. Care should be taken to count the antennomeres (11 in females, 12 in males) so that female specimens are not mistaken for males. Clavomeres that are only slightly wider than the preceding flagellomeres are more common, found in many species of the former Protelenomus and in more typical Trissolcus such as T. sipioides.
Trissolcus siliangae has a laterally invaginated metapostnotum, as in T. hullensis (
Trissolcus gajadanta, female (398371) A head, mesosoma, metasoma, dorsolateral view B head and mesosoma, posterolateral view C head, anterior view D head, mesosoma, metasoma, lateral view.
This species is named after one of its collectors, Dr. Siliang Wang, for her discovery of this species.
Four-gene maximum likelihood phylogenetic analysis of a modified dataset of
Holotype , female: China: Zhejiang, Wenzhou, corn field, 27.609301°N, 120.508985°E, phoretic on Cletus punctiger Dallas, 10.IX.2021, Cheng-jin Yan, SCAU 3042644 (deposited in SCBG). Paratypes: (3 females) China: 2 females, same data as holotype, SCAU 3042799, 3041198 (SCBG); 1 female, China: Zhejiang, Wenzhou, corn field, 27.609301°N, 120.508985°E, phoretic on Cletus punctiger Dallas, 10.IX.2021, Siliang Wang, SCAU 3044000 (WVCST).
China (Zhejiang).
The phenomenon of phoresy has been documented in a variety of scelionids: Paratelenomus anu Rajmohana, Sachin & Talamas (
This study was supported by the National Natural Science Foundation of China (31900346, 32100360), the Major Scientific and Technological Innovation Project of Wenzhou (ZN2019002), the Wenzhou Agricultural Harvest Found (FSJH2021004), the Science and Technology Commissioner Project of Wenzhou (X20210058) and the Wencheng County’s Second Phase of Innovation and Entrepreneurship Seed Fund (2019NKY09). We thank Lubomir Masner (CNCI) for generously donating a specimen of Trissolcus gajadanta and Natalie McGathey (FDACS/DPI) for photographing it. Zachary Lahey is a participant of the Oak Ridge Institute for Science and Education (ORISE) Agricultural Research Service (ARS) Research Participation Program, supported by the USDA-ARS U.S. Vegetable Laboratory in Charleston, SC, USA. Elijah Talamas was supported by the Florida Department of Agriculture and Consumer Services, Division of Plant Industry. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA.
Sequenced taxa and GenBank accession numbers
Data type: table