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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Methods
    • Leaf-cutting ant nest collection and immune challenge preparation
    • Experiment 1: Behavioral response to M. anisopliae exposure
      • Self-grooming
      • Allogrooming
      • Fungus grooming
      • Metapleural gland grooming
      • Fecal fluid grooming
    • Experiment 2: Worker’s survival and colonization by M. anisopliae
    • Experiment 3: Gene expression associated with innate immunity in workers
    • RNA extraction and relative gene expression
    • Statistical analyses
  • Results
  • Discussion
  • Conclusion
  • Acknowledgements
  • References
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